火把花根对大鼠系膜增殖性肾炎MCP－1表达的影响

A influence of MCP-1 of pathogenesis using colquhoumia root in MsPGN

目的观察火把花根及泼尼松对系膜增殖性肾炎（MsPGN）大鼠模型的干预，比较两药的疗效，并探讨其作用机制。方法将60只大鼠随机分成模型组、火把花根组、泼尼松组和对照组。于干预后第2周末测量24h尿蛋白及肝肾功能，观察肾脏病理改变，并用RT-PCR及免疫组化方法检测肾组织中羊抗人单核细胞趋化因子（MCP-1）的mRNA及蛋白质表达。结果结果MsPGN模型组大鼠尿蛋白定量、肾小球系膜相对面积及肾组织内MCP-1的mRNA及蛋白质表达均显著上调（P〈0．01）。药物干预后，上述上调指标均被抑制（P〈0．01）。火把花根组下调较泼尼松组更明显。结论火把花根或泼尼松均对MsPGN大鼠模型具有治疗作用；此疗效可能与下调肾组织MCP-1相关。

Objective To study the efficacy and the mechanism of Colquhoumia root （Tripteryglum hypoglaucum （Levl Hutch） and Prednisone in treatment of MsPGN. Method Wistar rats were injected with ATS to make MsPGN model. The rats were then randomly divided into 4 groups： nomal control, model group, col treated group and pre treated group. By 2 weeks, proteinuria level, liver and renal functions were measured. Histopathological, immunohistochemical , RT-PCR technique were used to evaluate the mesangial matrix production, the mRNA and protein expression of MCP-1 in tissue of kidney respectively. Results The proteinuria and the relative area of glomerular mesangium were increased significantly in model group. The expression of MCP-1 mRNA and protein in renal tissue were much higher in model group than control group （P d0. 01）. After treatment with Col or Pre, the proteinuria, the expression of MCP-1 mRNA and protein in renal tissue were significantly decreased in both treatment groups as compared with those in model groups renal. Col treated group was more significant than Pre treated group. Conclusions Co1 and Pre are effective in reducing proteinuria and mesangial matrix proliferation in MsPGN, and the effects may be achieved through down-regulation of expression of MCP-1 mRNA and protein in kidney.