荨麻水溶性化学成分研究

Study on water-soluble constituents of Urtica fissa

目的 研究荨麻的水溶性化学成分。方法 运用大孔树脂、聚酰胺柱色谱、硅胶柱色谱等方法对荨麻水溶性化学成分 进行分离;运用UV,IR,MS,NMR等方法进行结构鉴定。结果 从荨麻全草中分离并鉴定了7个化合物,分别为硝酸钾(potassi- um nitrate,Ⅰ)、异鼠李素(isorhamnetin,Ⅱ)、山柰酚-7-O-β-D-吡喃葡萄糖苷(kaempferol-7-O-β-D-glucopyranoside,Ⅲ)、槲皮素-7-O- β-D-吡喃葡萄糖苷(quercetin-7-O-β-D-glucopyranoside,Ⅳ)、异鼠李素-3-O-β-D-芸香糖苷(isorhamnetin-3-O-β-rutinoside,Ⅴ)、3,5-二 咖啡酰奎宁酸(3,5-di-O-caffeoylquinic acid,Ⅵ)、芦丁(rutin,Ⅶ)。结论 化合物Ⅱ-Ⅶ均为首次从荨麻中分得,其中Ⅳ,Ⅴ为首 次从荨麻属中分得。

OBJECTIVE To isolate and identify the chemical constituens in the water-soluable fraction of Urticafissa with anti-inflammatory and analgestic activity. METHODS Compounds were isolated by a combination of polyester, polyamide and silical gel colum chromatography, and their structures were identified by UV, IR, MS and NMR spectroscopic methods. RESULTS Seven known compounds were isolated from Urticafissa and identified as potassium nitrate（ Ⅰ）, isorhamnetin（Ⅱ）, kaempferol-7- O-β-D-glucopyranoside（Ⅲ）, quercetin-7- O-β-D- glucopyranoside（Ⅳ）, isorhamnetin-3- O-β-rutinoside （Ⅴ）, 3,5-di- O-caffeoylquinic acid （ Ⅵ）, rutin （ Ⅶ ）. CONCLUSION Compound Ⅳand Ⅴare isolated from genus Urtica for the first time,compound Ⅱ , Ⅲ ,Ⅵ and Ⅶ are obtained from Urticafissa for the first time.