离子对色谱法测定乙酰谷酰胺铝的含量与有关物质

Quantification of aceglutamide aluminum and its related substances by ion pair chromatography

目的:建立离子对色谱法测定乙酰谷酰胺铝(化学名:N-乙酰-L-谷氨酰胺铝复合物)的含量与有关物质.方法:采用Diamonsil C18柱(150 mm×4.6 mm,5 μm),流动相为甲醇-水(含4 mmol·L-1四丁基氢氧化铵与30mmol·L-1磷酸二氢钾,磷酸调pH 5.0)(1:100),柱温:室温,流速:1 mL·min-1,检测波长210 nm,进样量20 μL.结果:乙酰谷酰胺铝的线性范围为20～70 μg·mL-1(r=0.999 9),平均回收率为99.89%,RSD为1.1%.结论:本法操作简便,专属性好,适合于测定乙酰谷酰胺铝的含量与有关物质.

Objective： To establish an ion pair chromatography aluminum （N-acetyl-L-glutamine aluminium complex ） and its for the determination related substances. of aceglutamide Methods： The chromatography conditions were as followed： a Diamonsil C18 column（150 mm ×4.6mm,5μm） with room temperature; the mobile phase composed of methanol-water （1：100） containing 4 mmol·L^-1 tetrabutylammonium hydroxide and 30 mmol·L^-1 potassium dihydrogen phosphate （pH 5.0 adjusted with phosphoric acid）; flow rate at 1 mL·min^-1; the detection at 210 nm and the sample loading volume of 20μL. Results： The calibrated linear curve of aceglutamide aluminum concentration was in the range of 20 - 70 μg·mL^-1 （ r = 0.999 9）.The average recovery was 99.89% and RSD was 1.1%. Conclusion： The specific method is used for the quantification of aceglutamide aluminum and its related substances.