针刺大鼠“四白”传入信息对孤束核神经元放电的影响

Effect of Acupuncture at "Sibai"(ST 2),etc.on Discharges of Neurons of Nucleus Tractus Solitarii in the Rat

目的:比较足阳明经中远近腧穴及手太阳小肠经腧穴和非经非穴点的传入信息在孤束核(NTS)的差异。方法:采用细胞外记录方法,以足阳明胃经“四白”穴为刺激点,并以同名经的“地仓”和“内庭”、手太阳小肠经的“颧”、非经非穴“四白”旁开点为对照,在刺激点上用手针施以捻转手法刺激30 s,在NTS寻找对来自体表刺激点有反应的神经元。结果:52只大鼠记录到资料完整的86个NTS神经元中,针刺“四白”后NTS有反应神经元出现的机率(含兴奋和抑制性神经元)为65.12%,而针刺“地仓”“内庭”“颧”“四白”旁开点有反应神经元出现的机率分别为50.00%、46.51%、34.88%、31.40%。统计表明,针刺“四白”后NTS有反应神经元出现的机率均极显著地高于针刺“地仓”“内庭”“颧”“四白”旁开点后有反应神经元出现的机率(P<0.01);针刺“地仓”和“内庭”后NTS有反应神经元出现的机率均显著高于刺激“颧”和“四白”旁开点的有反应神经元出现机率(P<0.05)。针刺上述穴和非穴区,NTS神经元主要是以兴奋为主。针刺“四白”“地仓”“内庭”“颧”“四白”旁开点,其兴奋性神经元频率变化率分别为(35.08±4.80)%、(28.25±5.46)%(、27.57±4.87)%、(20.02±4.23)%(、18.55±2.49)%,“四白”与各组之间有显著性或极显著性差异(P<0.05或P<0.01),“地仓”“内庭”与“颧”“四白”旁开点亦有显著性或极显著性差异(P<0.05或P<0.01)。结论:与手太阳小肠经穴以及非经非穴点比较,足阳明经穴与NTS的关系更密切;足阳明经中远近腧穴的信息传入在NTS亦存在着一定的差异,这提示腧穴均有一定的特异性。

Objective： To compare the difference of effects of acupuncture （Acu） at different acupoints and non-acupoint on the electrical activities of neurons in rat nucleus tractus solitarii （NTS）. Methods： A total of 52 Wistar rats anesthetized by 10% urethane （1 mL/100 mg） were used in the present study. Extracellular discharges of NTS neurons were recorded by using glass micropipettes （about 1 μm in diameter, 10-30 MΩ） and microeleotrode amplifier, and analyzed by Power-Lab Data Acquisition-analysis System. “Sibai”（ST 2）, “Dicang”（ST 4）, “Neiting”（ST 44）, “Quanliao”（SI 18） and non-acupoint （0.5 cm superior-lateral to ST 2） were punctured respectively and manipulated with hand. Results： After acupuncture stimulation, of the 86 NTSneurons recorded, the numer of the excitatory, inhibitory and no-reaction neurons were 37, 19 and 30 respectively for “Sibai”（ST 2） ; 22, 21 and 43 for “Dicang”（ST 4） ; 19, 21 and 46 for “Neiting”（ST 44） ; 20, 10 and 56 for “Quanliao”（SI 18） ; and 20, 7 and 59 for nomacupoint, with the reaction rates being 65.12%, 50.00%, 46.51%, 34.88%, and 31.40% separately. The rate of reaction neurons of acupuncture of ST 2 was significantly higher than those of acupuncture of the other acupoints （ST 4, ST 44, SI 18） and non-acupoint（ P〈 0.05, 0.01 ）, and those of acupuncture of ST 4 and ST 44 were significantly higher than those of SI 18 and non-acupoint （P〈0.05）. No significant differences were fcund between acupuncture of ST 4 and ST 44, and between SI 18 and non-acupoint （P〉0.05）. The increased rates of the excited NTS neurons of acupuncture of ST 2 and other acupoints and non-acupoint were similar to the changes of the reaction rates mentioned above （P〈0.05, 0.01 ）, displaying that the effect of the afferent information of acupuncture of ST 2 on the electrical activity of NTS neurons was markedly stronger than that of acupuncture of the other acupoints and non-acupoint. Conclusion： C, crnpared with acupoint of the Small Intestine （ SI ） Meridian and non-acupoint, acupoints of the Stomach （ST） Meridian, particularly ST 2, have a more intimate relation to NTS neurons, and there exists specificity among different acupoints.