葛根素对成骨细胞生物学作用的实验研究

Experimental study of the effects of puerarin on biological characters of osteoblasts in vitro

目的:观察葛根素对体外培养SD大鼠成骨细胞增殖、分化、矿化的影响。方法:取新生SD大鼠的颅骨,胶原酶法培养成骨细胞。应用MTT法,对硝基苯磷酸盐法(PNPP)及茜素红染色方法观察葛根素对体外培养成骨细胞的增殖、碱性磷酸酶活性及矿化结节形成的影响。结果:葛根素具有刺激成骨细胞增殖,提高碱性磷酸酶活性及矿化结节形成数量的作用(P<0.01或P<0.05)。结论:葛根素具有促进成骨细胞的增殖、分化及矿化的作用。

Objective： To observe the effect of puerarin on cell proliferation, differentiation, maturation and mineralization in cultured rat osteoblasts. Method： Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro. Result： It was found that puerarin had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts （ P 〈 0.01 or P 〈 0.05）. Conclusion： Puerarin can promote proliferation, differentiation, maturation and mineralization of the osteoblasts in vitro.