酸奶菌种冷冻干燥保护剂的筛选研究

Screening of the Proper Freeze-drying Protectants for Yoghurt Starter

本研究采用Plackett-Burman设计法,对蔗糖、麦芽糖、乳糖、果糖、葡萄糖、甘油、甘露醇、山梨醇、可溶性淀粉、明胶、抗坏血酸、谷氨酸钠、L-半胱氨酸盐酸盐以及脱脂奶粉等14种材料对德氏乳杆菌保加利亚亚种和唾液链球菌嗜热亚种冷冻干燥的保护效果进行评价。结果表明:对德氏乳杆菌保加利亚亚种在冷冻干燥中有显著保护作用的是蔗糖、甘油、山梨醇以及脱脂奶粉;而唾液链球菌嗜热亚种有效的冷冻干燥保护剂为甘油、谷氨酸钠和脱脂奶粉。

Plackett-Burman design was used to evaluate the freeze-drying protective effect of sucrose, maltose, lactose, fructose, glucose, glycerol, manmtol, sorbitol, soluble starch, gelatin, ascorbic acid, sodium glutamate, L-cysteine hydrochloride and skim milk for yoghurt starter. Results showed that sucrose, glycerol, sorbitol and skim milk were the effective freeze-drying protectants for Lactobacillus delbrueckii subsp, bulgaricus, while glycerol, sodium glutamate and skim milk were the best freeze-drying protectants for Streptococcus sativarius subsp, thermophilus.