银杏不同部位中黄酮含量分析方法研究

Study on the Analytical Methods of the Ginkgo Flavonoid in Different Parts of Ginkgo biloba L

采用有机溶剂索氏提取法进行提取,利用紫外分光光度计、高效液相色谱等分析仪器对银杏叶、银杏果梗、银杏外种皮中银杏黄酮成分含量进行了检测。通过对比研究紫外分光光度法和高效液相色谱法对银杏不同部位黄酮含量的检测结果,得出准确高效的银杏黄酮含量的检测方法。结果发现银杏黄酮的含量用紫外分光光度计的检测结果普遍高于高效液相色谱的检测结果,而HPLC法相对干扰较小,重现性好,更能反映其内在质量,准确可靠。

The constrast research on the analytical methods of the flavonoid compounds in different parts of Ginkgo biloba L were studied by using Soxhlet＇s extracting method, spectrophotometer and HPLC were adopted to analyze the products. The Ginkgo flavoinoid contents assayed by the spectrophotometer and HPLC were compared so as to find a more accurate determination methods for Ginkgo flavoinoid. The result was that the content of Ginkgo flavoniod detected by spectrophotometer was generally higher than that of HPLC. But HPLC showed the advantages of little interference and good fidelity, and better expression of the immanent quality. So the HPLC was more rapid, accurate and reliable.