烟草SSR反应体系的优化

Optimization of SSR Reaction System in Tobacco

以烤烟品种NC2326、G28及其F1为材料,研究了烟草SSR分析中PCR反应体系的主要成分对SSR扩增结果的影响。结果表明:在总体积为20μL的PCR反应中,含20ng模板DNA,1.5U TaqDNA聚合酶,MgCl2终浓度为1.5mmol.L-1,dNTPs浓度为200μmol.L-1,引物浓度为0.25μmol.L-1时效果较好。

Using flue-cured tobacco cv. NC2326, G28 and their hybrid F1 as materials, the effects of major elements in PCR reaction system on SSR analysis were studied. The results showed that under the conditions of 1.5U TaqDNA polymerase, terminal MgCI2 concentration of 1.5 mmol·L^-1, 200μmol·L^-1 dNTPs, 0.25μmol·L^-1 primers and 20ng templates of DNA in total volume of 20μL, the result was better in PCR reaction.