β-榄香烯对脂质体瘤苗抗小鼠H_(22)肝癌的免疫增强作用

Enhanced Anti-H_(22) Hepatoma Effect of Liposomal Tumor Vaccine Modified by β-elemene

目的:制备榄香烯脂质体瘤苗,观察对抗小鼠H22肝癌作用,寻找有效的抗肝癌生物疗法。方法:用改进的机械分散法制备β-榄香烯修饰脂质体瘤苗,使磷脂酰胆碱∶胆固醇∶十八烷氨∶β-榄香烯摩尔比为4∶4∶1∶1.5,并通过腹腔接种将脂质体包裹的H22抗原(LA g组)及脂质体共包裹β-榄香烯与H22抗原的制剂(LEA g组)分别免疫荷瘤小鼠,观察小鼠的生存期,M TT法测定淋巴细胞对H22肝癌细胞的杀伤作用。结果:LEA g组的生存期和淋巴细胞毒活性均优于LA g组(P<0.05)。结论:β-榄香烯可增强脂质体瘤苗的抗肝癌免疫效应。

Objective：To observe the antitumor effect of liposomal tumor vaccine modified by β-elemene and look for the effective tumor vaccine formulation. Methods： MLVs （multilamellar vesicles） containing β-elemene and H22 Ags were prepared by improved mechanical dispersion with the lipid and β-elemene molar ratio ： PC（phosphal tidylcholine） ： CH（cholesterol） ： SA （stearylamine） ： β-elemene = 4 ： 4 ： 1 ： 1.5. H22 Ag entrapped in liposomes （LAg group）,liposome coentrapped with H22 Ag and β-elemene（LEAg group）were inoculated intraperitoneally into BalB/c mice loaded with H22 Hepatoma cells, respectively;the survival period were compared,the lymphocyte cytotoxicity was valued by MTT assay. Results ：The survival period was longer and lymphocyte cytotoxicity was stronger in LEAg group compared with that control （P〈0.05）. Conclusions：β-elemene can enhance the antitumor immune response of liposomal tumor vaccine.