实验性家兔缺血预适应对心肌缺血再灌注损伤的保护作用

Protective effect of ischemia preadaptation on myocardium injury of ischemic reperfusion in experimental rabbits

目的采用家兔心肌缺血再灌注损伤动物模型,观察缺血预适应对心肌缺血再灌注损伤的心肌保护作用。方法将家兔随机分成3组,即假手术组、缺血再灌注组、缺血预适应组,观察血清心肌酶学变化及心肌细胞的超微结构。结果(1)缺血预适应组心肌酶水平与缺血再灌注组相比差异均极为显著(P<0.01);(2)缺血预适应可明显改善亚细胞结构损害。结论缺血预适应可以降低心肌酶活性,缩小梗死面积,改善亚细胞结构损害,对心肌缺血再灌注损伤有保护作用。

Objective To observe the protective effect of ischemic preadaptation on myocardium injury of ischemia reperfusion by adopting the animal model of ischemia reperfusion on myocardium injury rabbits. Methods Rabbits were divided randomly into 3 groups （namely non-operation group,ischemia reperfusion group and ischemia preadaptation group） in order to observe the level of myocardium enzymatic dynamics and myocardium subcellular structure. Results （1）Level of myocardium enzyme with ischemia reperfusion group is significantly different compared to ischemia preadaptation（ P 〈 0.01 ）. （2） Ischemia preadaptation can evidently reduce subcellular structure injury. Conclusions Ischemia preadaptation can protect myocardium injury in ischemia reperfusion,the protection is mainly reflected by reduction of myocardium enzyme activity,myocardium infarct area and subcellular structure injury.