摘要
为了能够对传染性软疣病毒(MCV)感染做出准确快速的实验室诊断,从14例临床MCV患者皮疹处提取获得病毒DNA,设计引物并进行PCR扩增获得预期的167bp的片段,经测序并与已报道的序列比对,完全一致。而使用痘病毒科其他病毒的特异引物(正痘病毒和副痘病毒属)则没有特异扩增条带。将病毒DNA进行系列稀释后行PCR扩增,结果表明PCR检测方法的敏感性为5×10-4ng/μL。
Molluscum contagiosum virus （MCV） is associated molluscum contagiosum in human. A PCR assay was developed in our lab for rapid detection and identification of MCV infection. The primers used were designed to amplify a 167 bp of MCV genome. Others primers, designed to amplify gene of orthopox virus or parapoxvirus, were also used in this assay. The specificity and sensitivity of this assay were evaluated using several clinical specimens. As little as 5×10^-4ng/μL could be detection in this assay; and sequencing of PCR products confirmed the complete homology to MCV genome published. This assay may provide a highly sensitive means of rapid diagnosis of MCV infection in clinical materials.
出处
《生物技术通讯》
CAS
2005年第6期635-637,共3页
Letters in Biotechnology
基金
国家科技攻关计划项目(2003BA712A01)
关键词
传染性软疣病毒
PCR
诊断
molluscum contagiosum virus
polymerase chain reaction
diagnosis
