乙型肝炎肝组织血管内皮生长因子的表达变化

Expression change in vascular endothelial growth factor in hepatic tissues of hepatitis B patients

目的研究血管内皮生长因子(VEGF)表达与乙型肝炎(HB)肝血管增生及肝纤维化的关系。方法从人肝组织提取VEGF总RNA,经RTPCR扩增取得VEGFmRNA基因探针,对160例HB肝组织(HB组)及10例正常肝组织(对照组)的VEGFmRNA作了定位观察,同时对VEGF表达变化进行了检测。结果原位杂交显示,对照组VEGFmRNA阴性。HB组VEGFmRNA定位于肝窦、狄氏腔及扩张的肝窦周围肝细胞胞质;而VEGF则有胞质、膜窦、窦内皮3种形态,其表达强度、分布范围与HB分级分期、肝血管炎症、破坏、阻塞、增生及纤维化呈同步关系,不同肝病变之间差异显著(P<0.01)。结论肝组织VEGF表达增强可促进肝血管生成及肝窦毛细血管化。

Objective To study the relationship between vascular endothelial growth factor （VEGF） expression and proliferation of hepatic blood vessel and fibrosis in hepatitis B （HB） patients. Methods The total RNA of VEGF was extracted from human liver tissues, and VEGF mRNA probe was acquired by RT-PCR. It was then labeled on hepatic tissues of 160 patients with HB and 10 healthy individuals （control group）. Immunohistochemistry of VEGF was performed at the same time. Results The results of hybridization in situ showed that VEGF mRNA was negative in the control group. While in the HB groups, VEGF mRNA was located in the hepatic sinusoids, Disse＇s space and hepatocyte cytoplast around the dilated sinusoids, Immunohistochemistry showed thai VEGF was expressed in three patterns： the cytoplasm, sinusoid membrane, and sinusoidal endothelium. The expression strength and distribution range of VEGF were closely related with the grading and staging of HB, hepatic vascular inflammation, destruction, obstruction, proliferation and fibrosis. There was remarkable difference between different liver pathological changes （P〈0.01）. Conclusion Up-expression of VEGF in liver tissues of HB can promote hepatic vascular proliferation and liver sinusoidal capillarization.