口服异丙酚对小鼠伤害性刺激抑制作用的实验研究

Inhibitory effects of oral propofol on nociceptive stimuli in mice

目的观察口服异丙酚对伤害性刺激的抑制作用及量效关系。方法用昆明小鼠福尔马林足底注射和0.6%醋酸腹腔注射复制疼痛模型,观察和记录口服不同剂量异丙酚(100、150、200、250mg/kg)对小鼠自发缩足和扭体次数的影响。结果口服异丙酚剂量为100mg/kg时,对福尔马林诱发的自发缩足反射及醋酸诱发的扭体反应次数无明显影响,增加剂量后,自发缩足反射及扭体反应次数明显减少(P<0.05)。结论口服异丙酚对福尔马林和醋酸化学刺激具有抑制作用。

Objective To investigale whether oral propofol has any inhibitory effects and dose-response relationship on the pain inducing tissue injury in mice. Methods The effect of propofol on pain was observed in formalin test and acetic acid writhing test in mice. Formalin was injected subcutaneously into the plantar surface of one hind paw. Spontaneuus nocuous responses were immediately scored by counting the number of flinches of the injected hindpaw at every 5 minute interval during a 60-minute period. The number of writhing caused by intraperitoneal injection of 0. 6% acetic acid was also observed in mice. Results Oral propofol in a dose of 100mg/kg did not significantly inhibit nocuous stimulation. With higher doses, propofol inhibited both the phases 1 and 2 of persistent spontaneous pain induced by subcutaneous formalin injection. Orally taken propofol also inhibited the number of writhing after intraperitoneal acetic acid injection in a dose dependent manner. Conclusion Oral propofol is effective in inhibiling pain induced by formalin and acetic acid.