塞来昔布对非小细胞肺癌细胞增殖的抑制作用

The inhibitory effect of celebrex on non-small cell lung cancer

目的研究塞来昔布对非小细胞肺癌(NSCLC)的抑制作用及机制。方法应用MTT法检测塞来昔布对NSCLC细胞株的体外抑制作用,应用流式细胞学、透射电镜研究塞来昔布对NSCLC细胞株的细胞周期阻滞和诱导凋亡的作用,应用RTPCR检测P27KIP1、XIAP的表达,以研究细胞周期阻滞和诱导凋亡的机制。结果MTT比色试验表明,塞来昔布对NSCLC有明显的抑制作用,且表现为剂量依赖性和时间依赖性;而且,在相同条件下塞来昔布对NCIH520的抑制率明显高于对A549的抑制率。流式细胞学试验证明,塞来昔布作用后G0/G1细胞比例上升,细胞阻滞于G0/G1期。流式细胞学、透射电镜证实了凋亡的存在,而且凋亡率随剂量的增加而增加。RTPCR证实塞来昔布作用后P27KIP1mRNA表达增加,XIAPmRNA表达减少。结论塞来昔布在体外对NSCLC有明显的抑制增殖作用,其机制涉及细胞周期阻滞、诱导细胞凋亡两个方面。

Objective To investigate the inhibitory effect of celebrex on non-small cell lung cancer and its mechanisms. Methods the inhibitory effect of celebrex on NSCLC were detected by MTT Flow cytometry, electron microscope were used for evaluation of apoptosis and cell cycle block. The expression of P27^KIP1, XIAP were detected by reverse transcription-polymerase chain reaction. Results 1. celebrex inhibited cell survival of NSCLC in a time-dependent and concentration-depended manner, and the effect of celebrex were more pronounced in NCI-H520 than in A549. 2. Flow cytometry show that celebrex induced a G0/G1 cell arrest in NSCLC. 3. The result of these apoptosis test indicate that celebrex caused apoptosis in concentration-depended manner. 4. celebrex increased the expression of P27^KIP1 ,and decreased the expression of XIAP. Conclusions celebrex inhibited cell survival of NSCLC. Its mechanisms involved in cell cycle arrest and apoptosis.