APE1在鼻腔NK/T细胞淋巴瘤中的表达及其意义

Expression of APE1 gene in nasal NK/T cell lymphoma and its significance

目的探讨DNA损伤修复基因脱嘌呤/脱嘧啶核酸内切酶(APE1)在鼻腔NK/T淋巴瘤中的表达及其临床意义。方法用免疫组化法对64例鼻腔NK/T细胞淋巴瘤和10例正常淋巴结中APE1的表达及定位进行检测,并通过图像分析系统计算其积分光密度值。同时应用免疫组化SP法和TUNEL法检测细胞增殖和凋亡变化。结果APE1在淋巴瘤中存在胞质、胞核和胞核/胞质表达,且以胞核表达明显,其中APE1阳性分度在复发/难治组、无复发/难治组、正常对照组鼻腔NK/T细胞中依次降低(P<0.01)。APE1高表达多伴随着细胞增殖升高和凋亡减少。APE1表达的程度和患者的预后密切相关。结论APE1基因表达强度与淋巴瘤的疗效有关,APE1基因表达增强可能是NK/T淋巴瘤患者预后不良的指标之一。

Objective To investigate the expression of DNA damage and repair gene Apurinic/apyrimidinic endonuclease （APE1） prorein in nasal NK/T cell lymphoma, and elucidate its clinical implication. Methods Expression of APEI proteins was detected immunohis-tochemically in 10 normal lymph nodes and human nasal NK/T cells from lymphoma of 64 patients and their integral optical density was determined by means of image analytic system. The proliferation index and apoptosis index were determined by means of immunohisto-chemical staining and terminal dUTP nickend labeling （TUNEL） technique. Results 1. Nuclear, nucleus/cytoplasmic and cytoplasmic types of APEI positive staining could be noted in nasal NK/T cell lymphoma. Expression of APEI gene in nucleus was significantly strengthened compared to that in nucleus/cytoplasm and cytoplasm. In relapse or refractory group, no relapse or refractory group, and normal control group, the positive degree of cytoplasmic staining diminished significantly in the above order （P〈0.01）. 2. High expression of APEI was correlated with high proliferation index and low apoptosis index. 3. The degree of expression of APEI in nasal NK/ T cell lymphoma was closely related with the prognosis of the patients. Conclusion Expression degree of APE1 gene may be associated with nasal NK/T cell lymphoma treatment efficacy. High expression of APE1 gene may predict poor prognosis.