急性淋巴细胞白血病中hMSH2mRNA及突变型P53蛋白的表达

Abnormal Expression of hMSH2 mRNA and Mutation P53 Protein in Acute Lymphoblastic Leukemia

为了探讨hMSH2mRNA及突变型P53蛋白表达与急性淋巴细胞白血病发病的关系,采用寡核苷酸探针原位杂交技术及免疫细胞化学技术检测急性淋巴细胞白血病(ALL)患者骨髓单个核细胞的滴片hMSH2mRNA和突变型P53蛋白的表达。结果显示:ALL组hMSH2mRNA表达百分比低于对照组,分别为(32.88±11.46)%和(64.22±8.51)%,两组比较有显著性差异(P<0.05);ALL组突变型P53蛋白表达百分比高于对照组,分别为(29.25±9.45)%和(12.63±6.66)%,两组比较有显著性差异(P<0.05);ALL组、对照组中hMSH2的mRNA表达百分比与突变型P53蛋白表达百分比呈负相关(r值为-0.45,P<0.05)。结论:hMSH2基因表达缺失及P53蛋白突变可能参与ALL的发生和发展;hMSH2mRNA表达缺失与突变型P53蛋白的高表达共同促进了ALL的发生。

In order to investigate the mechanism of acute lymphoblastic leukemic cell malignant proliferation, the expressions of hMSH2 mRNA and mutation P53 （ mtP53 ） protein in bone marrow cells of de novo acute lymphoblastic leukemia （ALL） were determined by in situ hybridization and immunocytochemical methods. The results showed the that percentage of positive cell with hMSH2 mRNA expression was （32.88 ±11.46） % in the de novo ALL group and （64.22 ± 8.51 ）% in the control group. The percentage of positive cell with mtP53 protein expression was （29.25 ± 9.45 ） % in the de novo ALL group, and （ 12.63 ± 6.66 ） % in the control group. There was a significant negative correlation between the positive percentages of hMSH2 mRNA expression and mtP53 protein expression （ r = - 0.45, P 〈 0.05 ）. It is concluded that defective MSH2 mRNA expression plays an important role in the pathogenesis of acute lymphoblastic leukemia, mtP53 protein mutation plays an important role in the development of acute lymphoblastic leukemia, the hMSH2 mRNA defect can lead to accumulation of the mutant P53 protein in acute lymphoblastic leukemia, and both jointly promote the pathogenesis of ALL.