重组人粒细胞集落刺激因子对阿糖胞苷诱导HL-60细胞凋亡的影响

Effects of RhG-CSF on Apoptosis of HL-60 Cell Induced by Cytarabine

为了研究重组人粒细胞集落刺激因子(rhGCSF)对阿糖胞苷(AraC)诱导HL60细胞凋亡的影响,体外培养HL60细胞,加入rhGCSF和(或)AraC,瑞氏染色观察细胞形态变化,吖啶橙/溴化乙锭双染色观察细胞凋亡情况,MTT实验检测细胞增殖活性。结果表明:加入rhGCSF后AraC诱导的HL60细胞的凋亡率升高(P<0.01),而MTT实验的OD值降低(P<0.01)。结论:rhGCSF有诱导HL60细胞凋亡的作用,rhGCSF可以促进Arac诱导HL60细胞凋亡的作用。

To investigate the effects of rhG-CSF on apoptosis of HL-60 cell induced by cytarabine （ Ara-C）, HL-60 cell were cultured with Ara-C , with or without rhG-CSF, and the changes of HL-60 cells were detected by morphology, including Wright＇ s and acridine orange （AO） and ethidium bromide （EB） staining, and MTT assay. The results showed that comparing with Ara-C group, the apoptosis level of rhG-CSF ＋ Ara-C group was enhanced （ P 〈 0 . 01 ）, and the OD value of MTT assay declined（ P 〈0 . 01 ）. It is concluded that rhG-CSF can induce the apoptosis of HL-60 cells and enhance the apoptosis induced by Ara-C.