Rhesus盒检测技术

A Method for Rhsesus Box Test

为了研究Rhesus盒的检测技术及意义,根据RHD基因上游盒、下游盒及杂交盒的DNA序列特异性设计引物,用PCRSSP和错配PCR技术检测上游、下游和杂合的Rhesus盒。结果表明:DNA标准品验证本技术可靠,随机非血统关系RhD阳性者中,RHD+/RHD-型占9.00%,RHD+/RHD+型占91.00%;RhD阴性者中RHD+/RHD-型占26.14%,RHD+/RHD+型占3.92%,RHD-/RHD-型占69.94%。结论:Rhesus盒检测技术可用于分析RhD单倍型基因结构,用于遗传、临床输血及新生儿溶血病等研究。

To study the method for Rhesus box test and its significance, the sequence specific primers of upstream, downstream and hybrid Rhesus boxes were designed according to RhD gene sequence; the upstream, downstream and hybrid Rhesus boxes were determined by PCP-SSP and mismatched PCR. The results showed that this method was confirmed by DNA Standard test. It was shown that in unrelative RhD positive individuals RHD^＋/RHD^- , RHD^＋/RHD^＋ genotype accounted for 9.00% , 91.00% respectively, and in RhD negative individuls RHD^＋/RHD^- , RHD^＋/RHD^＋, RHD^-/RHD^- genotype were 26.14% , 3.92%, 69.94% respectively. It is concluded that the method of Rhesus box test was confirned to be reliable and can be used for the identification of RhD haplotype gene structure, as well as for study on inheritance, clinical transfusion and neonatal hemolytic diseases.