单管PCR扩增鉴定ABO血型基因型

Determination of ABO Blood Group Genotypes with One Tube PCR Reaction

本研究目的是建立单管PCR扩增鉴定ABO血型基因型的方法。采用盐析法抽提DNA,应用单管PCR扩增结合基因扫描技术对ABO血型的基因型进行鉴定。结果表明:用本方法鉴定出的132例样本的ABO基因型结果与其血清学结果完全符合,A、B、O基因频率分别为0.205、0.159、0.636,其中AA基因型8例(6.1%),AO基因型31例(23.5%),AB基因型7例(5.3%),BB基因型6例(4.5%),BO基因型23例(17.4%),OO基因型57例(43.2%)。结论:单管PCR扩增结合基因扫描技术能鉴定ABO血型基因型。

This study was aimed to establish one tube PCR reaction technique to determine ABO blood group genotypes. Salting-out method was adopted to extract genomic DNA; one tube polymerase chain reaction with GeneScan technique was used to identify ABO genotypes. The results showed that the ABO genotypes of 132 samples were in accordance with the phenotypes determined by serological technique. The frequencies of A, B and O were 0.205,0. 159 and 0.636 respectively. AA, AO, AB, BB, BO and OO genotypes were 8（6.1% ）, 31 （23.5%）, 7（5.3% ）, 6（4. 5% ）, 23（ 17. 4% ）, and 57 （43. 2% ） respectively. It is concluded that one tube polymerase chain reaction with GeneScan technique can determine the genotyoes of ABO blood group.