摘要
RNA干扰(RNAi)是一种封闭基因表达的有效方法,通过小干扰RNA片段(siRNA)启动细胞内的RNAi反应,显著增加对目标mRNA的表达抑制。本文综述了RNAi机理,哺乳动物细胞siRNA靶点选择,以及siRNA载体构建和转染哺乳动物体细胞的研究现状。
RNA interference （RNAi）, a highly conserved evolutionary process of post-transcriptional gene silencing, can be triggered by small interfering RNAs （siRNAs） that mediate sequence-specific mRNA degradation. The article summarized some aspects of the mechanism of RNAi, siRNA design and delivery of siRNAs to mammalian somatic cells. And some hurdles in practice were also discussed.
出处
《中国实验血液学杂志》
CAS
CSCD
2005年第6期1141-1144,共4页
Journal of Experimental Hematology
基金
国家自然科学基金委海外青年学者合作基金资助项目
编号30228011
