小麦TaMlo基因的原核表达、抗体制备及细胞化学分析(简报)

PROKARYOTIC EXPRESSION, ANTISERUM PREPARATION AND CYTOCHEMICAL ANALYSIS OF TaMlo3 IN WHEAT

白粉病菌(Blumeria graminis)是一类高度专化性的寄生真菌,可侵染650多种单子叶植物和9000多种双子叶植物[1],能够引起多种麦类作物的白粉病,给农业生产带来巨大的损失.由于白粉病菌生理小种多、变异快,所以利用专化性抗病基因难以解决植物的持久抗病性问题.

Mlo gene in wheat is critical to determine wheat broad spectrum disease resistance against the pathogenic powdery mildew fungus. According to wheat TaMlo3 cDNA sequence, the two DNA fragments encoding the intracellular loop 2 and C-terminus of wheat Mlo protein were cloned respectively into the vector pET-30a, and then the fusion polypeptides were expressed in E. coli BL21. The recombinant proteins were purified by Ni^2＋-NTA agarose column, and then were used to immunize the rabbits respectively. Two polyclonal antisera were obtained and they could be specifically bound on the extrahaustorial membrane and extrahaustorial matrix of the powdery mildew fungus by immuno-gold labeling.