卡维地洛对TNF-α诱导心肌细胞凋亡的保护作用

Protective effects of carvedilol on myocardium cells apoptosis induced by TNF-α

目的:观察肿瘤坏死因子α(TNFα)诱导正常SD大鼠乳鼠心肌细胞凋亡的情况及卡维地洛对TNFα诱导心肌细胞凋亡的保护作用。方法:对实验SD大鼠乳鼠心肌细胞进行培养并分为3组:正常对照组,仅用DMEM培养液培养;TNFα组,TNFα加入DMEM培养液;卡维地洛组,DMEM培养液+TNFα+卡维地洛共同培养。分别用透射电镜、TUNEL法和流式细胞仪观察3组心肌细胞的凋亡情况。结果:TNFα组心肌细胞凋亡的发生率明显高于正常对照组(P<0.01);而卡维地洛组凋亡的发生率显著低于TNFα组(P<0.01)。结论:卡维地洛对TNFα诱导的心肌细胞凋亡有良好的保护作用。

AIM： To explore normal myocardium eells apoptosis induced by tumor necrosis factor-α（TNF-α） and the proteetive effects of earvedilol on it. METHODS： 1-3 davs SD rats myocardium cells were divided into three groups ： control group ： nomlal DMEM ; TNF-α group ： normal DMEM ＋ TNF-α; carvedilol group ： nomlal DMEM ＋ TNF-α ＋ carvedilol. We analyzed cell apoptosis by TUNEL and electron microscope and floweytometty.RESULTS： In.TNF-α group, apoptotie cardiomyocyte increased apparently compared with control group （P 〈 0.01）. But in carvedilol group, apoptotic cardiomyocyte decreased obviously compared with TNF-α group （P 〈 0.01 ）. CONCLUSIONS： Carvedilol has good protective effects on myocardium cells apoptosis induced by TNF-α.