两种泥鳅RAPD标记遗传稳定性分析

The Genetic Stability of RAPD Marker in Two Species of Loaches

用从20个随机引物中筛选出的7个引物对泥鳅(Misgurnus anguillicaudatus)和大鳞副泥鳅(Paramisgurnus dabryanus)及其自交与杂交子代进行了RAPD遗传稳定性分析。结果表明,RAPD谱带主要呈孟德尔式遗传,该类带纹明亮稳定,在泥鳅子代中占99.11%;在大鳞副泥鳅子代中占100%;在杂交子代中占99.36%。也存在非孟德尔式遗传谱带,该类带纹较暗、稳定性差,在泥鳅子代中为0.89%;在杂交子代中为0.64%;在大鳞副泥鳅中未见此类带纹。实验结果说明RAPD谱带具有很高的遗传稳定性。

The genetic stability of RAPD marker was analyzed in adult inbred or hybrid progenies of Misgurnus anguiUicaudatus and Paramisgurnus dabryanus by using 7 primers selected from 20 random primers. The results showed that the major RAPD stripes accorded with Mendelian inheritance. The bright and steady stripes represented 99.11% in M. anguillicaudatus inbred progenies, 100% in P. dabryanus inbred progenies, and 99.36% in hybrid progenies. And there were also abnormal segregations as shown in weak and unstable bands in low frequency, 0.89 % in inbred progenies of M. anguillicaudatus, and 0.64% in hybrid progenies. These results indicate that the genetic stability of RAPD marker is very high in loaches.