运动对高血压大鼠纤溶功能的影响

Effect of exercise on fibrilytic function of hypertension rats

目的:观察原发性高血压大鼠运动后血清内皮素、组织型纤溶酶原激活物和纤溶酶激活物抑制剂的变化,寻找运动降低原发性高血压大鼠血压的机制,为高血压患者制定运动处方提供理论依据。方法:实验于2003-07/09在扬州大学实验动物中心完成。采用原发性高血压大鼠18只作为实验动物,按实验需要将动物随机分为运动组(10只)和安静组(8只)。运动组大鼠开始进行1周适应性游泳,然后进行10周的训练,5d/周,60min/d的游泳运动。运动组游泳训练后,与安静组一起取静脉血,用发色底物法测定组织型纤溶酶原激活物和纤溶酶激活物抑制剂活性。结果:实验进行至第5周运动组死亡1只大鼠。共17只大鼠进入结果分析,运动组9只,安静组8只。原发性高血压大鼠经过持续的适量运动后,在第10周其组织型纤溶酶原激活物活性高于安静组犤(430±120),(280±77)IU/L,P<0.01犦,而纤溶酶激活物抑制剂活性明显低于安静组犤(310±30),(490±60)AU/L,P<0.01犦;组织型纤溶酶原激活物/纤溶酶激活物抑制剂则大大高于安静组(1.46±0.42,0.52±0.19,P<0.01)。结论:持续一定强度的运动不仅可以提高组织型纤溶酶原激活物,还可以降低纤溶酶激活物抑制剂1,即改善纤溶活性,从而减少血栓性并发症的发生。

AIM： To observe the changes of serum endothelins, tissue-type plasminogen activator （t-PA） and PAI-1 in rats with essential hypertension after exercise, search the mechanism of exercise on reducing blood pressure in rats with essential hypertension and provide theory basis for designing physical exercise prescription for hypertension patients. METHODS： The experiment was conducted at the experimental animal center of Yangzhou University between July and September 2003. Eighteen rats with essential hypertension were selected as experimental animals. According to the requirement of experiment, the animals were assigned randomly into exercise group （n=10） and quiet group （n=8）. The rats in the exercising group swam adaptively for 1 week, and then did training of swimming for 10 weeks, 5 days per week, 60 minutes per day. When the rats did training of swimming in the exercise group, the venous blood of rats was gained together with those in the quiet group. The activities of t-PA and PAI-1 were detected with assay of chromophorous substrate. RESULTS： One rat died at the 5^th week in the exercise group. Totally 17 rats were involved in the result analysis, 9 in the exercise group and 8 in the quiet group. After continuously proper exercise in rats with essential hypertension, the activity of t-PA at the 10^th week was higher than that in the.quiet group [（430±120）, （280±77）IU/L,P 〈 0.01], while activity of PAI-1 was lower markedly than that in the quiet group [（310±30）, （490 ±60）AU/L,P 〈 0.01]. t-PA/PAI-1 was higher significantly than that in the quiet group （ 1.46±0.42,0.52±0.19 ,P 〈 0.01 ）. CONCLUSION： The proper intensity of training not only can enhance the activity of t-PA, but also decrease PAl-1, i.e., improve fibrilytic activity so as to reduce thrombotic complication.