运动对大鼠骨矿盐含量的影响

Effect of exercise on bone mineral content of rats

目的:分析运动对正常成年大鼠和切除双侧卵巢后的大鼠骨矿盐代谢的影响。方法:本实验于2002-10/2003-05在广东医学院生理学教研室完成。普通级4月龄SD雌性大鼠30只。随机分成5组:正常对照组;正常大鼠+运动组;假去卵巢组;去卵巢组;去卵巢+运动组。然后重复,每组6只。正常大鼠+运动组和去卵巢+运动组大鼠于术后第7天开始进行运动训练,5d/周,45min/d,16m/min,跑道倾角0°,持续10周。第10周末,麻醉状态下动脉放血处死各组大鼠,观察骨干重、骨干重/体质量、骨灰重、骨灰重/体质量、骨灰重/骨干重(%)代谢变化。结果:30只大鼠均进入结果分析。大鼠骨干重、骨干重/体质量、骨灰重、骨灰重/体质量、骨灰重/骨干重(%)各指标代谢变化:正常大鼠+运动组各指标均显著高于正常对照组犤(645±48),(567±32)mg;(1.97±0.06),(1.73±0.10)g/kg;(440±30),(374±21)mg;(1.35±0.04),(1.14±0.06)g/kg;(68.2±0.7)%,(65.9±0.9)%,t=3.46～6.92,P<0.01犦;去卵巢组各指标均明显低于假去卵巢组犤(504±24),(569±40)mg;(1.55±0.61),(1.83±0.19)g/kg;(293±14),(381±23)mg;(0.90±0.04),(1.21±0.14)g/kg;(58.6±0.8)%,(66.3±1.5)%,t=3.64～15.58,P<0.01犦;去卵巢+运动组各指标均较去卵巢组明显增加(627±70),(504±24)mg;(1.76±0.11),(1.55±0.61)g/kg;(409±43),(293±14)mg;(1.15±0.08),(0.90±0.04)g/kg;(65.2±1.2)%,(58.6±0.8)%,t=3.90～11.21,P<0.01)。结论:中等强度运动是增加成年大鼠骨矿盐含量和预防去卵巢大鼠骨矿盐丢失的有效措施。

AIM： To analyze the effect of exercise on bone mineral metabolism of normal adult and bilaterally ovafiectomized rats. METHODS： The experiment was carried out in the Department of Physiology, Guangdong Medical College between October 2002 and May 2003. Thirty 4-month-old female Sprague-Dawley （SD） rats of common degree were randomly divided into 5 groups with 6 rats in each group： normal control group, normal exercise group, sham-ovariectomized group, ovariectomized group and exercise after ovariectomy group. The rats in the normal exercise group and exercise after ovariectomy group received exercise training from the 7s day after operation, 5 days a week for 10 weeks, 45 minutes per day, 16 m per minute, the dip of the runway was 0°. All the rats were killed by arterial blood letting under anesthesia at the end of the 10th week, the metabolic changes of bone dry mass, bone dry mass/body mass, bone ashes mass, bone ashes mass/body mass, bone ashes mass/bone dry mass （%） were observed. RESULTS： All the 30 rats were involved in the analysis of results. The metabolic changes of bone dry mass, bone dry mass/body mass, bone ashes mass, bone ashes mass/body mass, bone ashes mass/bone dry mass were significantly higher in the normal exercise group than in the normal control group [（645±48）, （567±32） mg; （1.97±0.06）, （1.73±0.10） g/kg, （440±30）, （374±21） mg; （1.35±0.04）, （1.14±0.06） g/kg; （68.2±0.7）%, （65.9±0.9）%, t=3.46-6.92, P 〈 0.01], but obviously lower in the ovariectomized group than in the sham-ovariectomized group [（500±24）, （569±40） mg; （1.55 ±0.61）, （1.83±0.19） g/kg; （293±14）, （381±23） mg; （0.90±0.04）, （1.21 ±0.14） g/kg; （58.6±0.8）%, 66.3±1.5]%, t=3.64-15.58, P 〈 0.01], and were markedly increased in the exercise after ovariectomy group as compared with the ovariectomized group [（627±70）, （500±24）mg; （1.76±0.11）, （1.55 ±0,61） g/kg; （409±43）, （293±14） mg; （1.15±0.08）, （0.90±0.04） g/kg; （65.2±1.2）%, （58.6±0.8）%; t=3.90-11.21, P 〈 0.01]. CONCLUSION： Exercise of moderate intensity is an effective method to increase the bone mineral content in adult rats and preventive the bone mineral loss in ovariectomized rats.