骨质疏松症大鼠白细胞介素6、骨钙素和骨碱性磷酸酶的表达特征(英文)

Expression properties of interleukin-6, osteocalcin and bone alkaline phosphatase in osteoporosis rats

背景:白细胞介素6、骨钙素和骨碱性磷酸酶均参与骨代谢过程,其与骨质疏松的发生有相关性。目的:观察白细胞介素6、骨钙素和骨碱性磷酸酶在骨质疏松大鼠中的表达特征。设计:随机分组观察对比实验。单位:深圳市人民医院(暨南大学医学院第二附属医院)骨科。材料:实验于2002-01/2003-01在深圳市人民医院动物实验室及中心实验室完成。6月龄雌性SD大鼠60只,平均体质量250g,随机分为3组:去势组,对照组,空白组,每组20只。方法:去势组手术切除大鼠双侧卵巢建立骨质疏松动物模型;对照组开腹后将卵巢提出腹腔后还纳关腹;空白组不行任何处理。分别于术后2,3,4,5,6个月后每组随机抽出大鼠4只,进行骨密度、骨钙素、白细胞介素6及碱性磷酸酶检测。主要观察指标:大鼠全身骨密度、血清骨钙素、血清白细胞介素6及血清骨碱性磷酸酶变化。结果:60只大鼠均进入结果分析。①大鼠骨密度测量结果:4,5,6个月去势组骨密度较对照组和空白组明显下降犤4个月:(0.1395±0.0078),(0.1470±0.0008),(0.1459±0.0029)g/cm2,5个月:(0.1379±0.0009),(0.1456±0.0008),(0.1447±0.0005)g/cm2,6个月:(0.1226±0.0004),(0.1450±0.0021),(0.1440±0.0009)g/cm2,P<0.05或<0.01犦。②大鼠血清骨钙素测量结果:4个月后去势组骨钙素显著高于对照组与空白组(P>0.05)。③大鼠白细胞介素6检测结果:各时间点去势组白细胞介素6均显著高对照组与空白组(P>0.05)④大鼠血清骨碱性磷酸酶:4,5,6个月去势组骨碱性磷酸酶显著高于对照组与空白组犤(2026±4)比(1247±12),(1291±7)nkat/L,(2342±9)比(1273±18),(1342±12)nkat/L,(2633±15)比(1340±9),(1357±8)nkat/L,P<0.05犦结论:在骨质疏松症大鼠中骨密度显著降低,白细胞介素6、骨钙素和骨碱性磷酸酶在骨质疏松症大鼠中有显著表达,可作为骨质疏松症的诊断及筛查指标。

BACKGROUND： Interleukin-6 （IL-6）, osteocalcin （OC） and bone alkaline phosphatase （BALP） are involved in bone metabolism, which is correlative with ＇osteoporosis. OBJECTIVE： To observe expression properties of IL-6, OC and BALP in rats with osteoporosis. DESIGN： Control experiment with observation of randpmized group division was designed. SETTING： Orthopedics Department of Shenzhen People＇s Hospital （2nd Affiliated Hospital of Medical College of Jinan University） MATERIALS： The experiment was performed in Animal Laboratory Room and Center Laboratory Room of Shenzhen People＇s Hospital from January 2002 to January 2003, in which, 60 SD female rats of 6 months were employed, averagely weighted 250 g. They were randomized into 3 groups, named ovarieetomized （OVT） group, control and blank group, 20 rats in each. METHODS： In OVT group, the ovaries of rat were removed bilaterally to establish osteoporosis model. In the control, after the ovary was lifted out of abdominal cavity when the abdomen was opened, it was returned back and the abdomen was closed. In blank group, no any management was performed. In 2, 3, 4, 5 and 6 months after surgery, 4 rats were randomized from each group for assays of bone density, OC, IL-6 and BALP. MAIN OUTCOME MEASURES： Changes of bone density, OC, IL-6 and BALP of whole body of rat. RESULTS： 60 rats all entered result analysis. （1） Result of bone density assay in rats： In 4, 5 and 6 months, in OVT group, bone density was reduced remarkably compared with the control and blank group [in 4 months： （0.139 5±0.007 8）, （0.147 0±0.000 8）, （0.145 9±0.002 9） g/cm^2, in 5 months： （0.137 9±0.000 9）, （0.145 6±0.000 8）, （0.144 7±0.000 5） g/cm^2 in 6 months： （0.122 6±0.000 4）, （0.145 0±0.002 1）, （0.144 0±0.000 9） g/cm^2, P 〈 0.05 or 〈 0.01]. （2） Result of serum OC assay in rats： In 4 months, OC in OVT group was higher than the control and blank group （P 〉 0.05）. （3） Resuits of IL-6 assay in rats： At every time spots, IL-6 in OVT group was higher significantly than the control and blank group （P 〉 0.05）. （4） Resuits of BALP in serum of rats： In 4, 5 and 6 months, BALP in OVT group was higher significantly than the control and blank group [（2 026±4） vs （1 247±12）, （1 291±7） nkat/L, （2 342±9） vs （1 273±18）, （1 342±12） nkat/L, （2 633±15） vs （1 340±9）, （1 357±8） nkat/L, P 〈 0.05]. CONCLUSION： In the rats with osteoporosis, bone density is decreased significantly and IL-6, OC and BALP are significantly expressed, which can be taken as the indexes for diagnosis and screen of osteoporosis.