不等单蚤和缓慢细蚤吸血前后脂肪和非特异性酯酶的组织化学研究

Histochemistry of fat and nonspecific esterase in newly emerged and sucked adults of rat fleas Monopsyllus anisus (Rothschild) and Leptopsylla segnis (Schnherr)

采用组织化学和显微摄影及定量图像分析技术,研究了不等单蚤Monopsyllus anisus(Rothschild)和缓慢细蚤Leptopsyllasegnis(Sch nherr)在新羽化和吸血后发育过程中脂肪和非特异性酯酶的分布及活性。结果显示,两种新羽化蚤脂肪主要存在脂肪体中,非特异性酯酶则主要存在于唾液腺和神经链中。不等单蚤吸血后中肠脂滴增加,非特异性酯酶活性增强,随消化时间的延长脂滴逐渐减少,但不同消化时间非特异性酯酶活性差异不显著;而缓慢细蚤吸血后中肠未见或偶见少量脂滴,非特异性酯酶活性也有增强,但酶活性低于相同消化时间的不等单蚤。卵母细胞发育过程中脂滴增多,非特异性酯酶活性逐渐增强,且不等单蚤酶活性强于缓慢细蚤的。

With histochemistry techniques and image analysis of microscope photographs, the distribution and activity of fat and nonspecific esterase （n-E） in newly emerged and sucked adults of rat fleas, Monopsyllus anisus （Rothschild） and Leptopsylla segnis （Schonherr） were studied. The results indicated that in newly emerged fleas of the two species the fat was distributed mainly in fat body, while nonspecific esterase was distributed mainly in salivary glands and nerve chain. After blood meal and digestion, in M. anisus a great deal of fat drops appeared in the midgut and the fat drops decreased as digestion continued, and the activity of nonspecific esterase increased, but there was no significant difference in its activity in different digestion time; while in L. segnis, there was few fat drops in the midgut, and the activity of nonspecific esterase also increased, but its activity was lower than that in M. anisus in the same digestion time. During growing up of the oocytes, the fat drops and activity of nonspecific esterase increased, and the activity of nonspecific esterase in M. anisus was higher than that in L. segnis.