摘要
对过敏性疾病主要过敏原屋尘螨Dermatophagoides pteronyssinusⅠ类变应原(Der p1)进行了抗原定位研究。选用经表达和纯化的Der p1抗原蛋白,按常规方法免疫小鼠,分离血清获抗重组Der p1的抗体(Ⅰ抗),用抗鼠IgG荧光抗体为Ⅱ抗,屋尘螨经石蜡切片,在荧光显微镜下对其特异性变应原的定位进行观察。HE染色显示屋尘螨消化系统占据大部分体腔。组织免疫荧光发现屋尘螨Ⅰ类变应原主要定位于螨的中肠组织及肠内容物,而螨的生殖系统、排泄系统等脏器以及几丁质甲壳均呈阴性反应。据此认为屋尘螨Ⅰ类变应原存在于螨的肠内容物和中肠组织中。
The localization of Der pl in the body of the house dust mite, Dermatophagoides ptekronyssinus was studied. Der pl antibody as primary antibody was prepared by immunizing the mice with Der pl antigen. The fluorescent antimouse IgG antibody was used as secondary antibody. D. pteronyssinus was embedded in paraffin, sectioned, and then reacted with the primary and secondary antibody. The location of Der pl in the body was observed with fluoroscope. Some sections were stained with HE staining. A large part of body cavity of D. pteronyssinus was occupied by the digestive system as shown with HE staining. The Der p1 lallergen was mostly localized in the midgut and gut contents, while the cuticle and reproductive system showed negative reaction in the immunostaining.
出处
《昆虫学报》
CAS
CSCD
北大核心
2005年第6期833-836,共4页
Acta Entomologica Sinica
基金
国家"863"计划项目(2002AA214011)
国家自然科学基金项目(30471505
30271226)
广东省科技重大专项(2003A3080502)
深圳市科技计划资助项目(200218)
关键词
屋尘螨
小鼠
Der
P1
抗原定位
HE染色
免疫染色
Dermatophagoides pteronyssinus
mice
Der pl
antigenic localization
haematoxylin eosin staining
immunostaining
