细胞外灌流对骨骼肌细胞介电谱的影响

The Effect of Extracellular Perfusion on Dielectric Spectroscopy of Skeletal Muscle Cells

通过灌流的方法,探讨细胞外液对骨骼肌细胞介电谱的影响,应用非线性Co le-Co le公式的曲线拟合与数值计算,比较灌流后骨骼肌细胞介电谱参数,结果表明:(1)低频段介电常数Lε和高频段电导率hκ随着细胞外灌流液电导率的降低而减少;(2)细胞外灌流对骨骼肌细胞介电谱的特征频率(fC 1,fC 2)影响不大;(3)细胞外灌流主要影响骨骼肌细胞Δε″和tgδ的峰值。

The dielectric spectroscopy of the skeletal muscle cells is approached by extracellular perfusion, using curve fitting and numerical calculation with nonlinear Cole-Cole equation, and the dielectric parameter of skeletal muscle cells is compared after perfusion, The results show： （1） the permlttivy at low frequency（εl） and the conductivity at hight frequency （κh） reduces in keeping with extracellular perfusate; （2） the effect of extracellular perfusion on the characteristic frequency （fc1,fc2） is small; （3） the extracellular perfusion mostly influences the peaks of Δε＂ and tgδ for the skeletal muscle cells.