异鼠李素对Eca-109细胞DNA的合成和c-myc基因表达近日节律的影响

Effect of Isorhmnetin on Circadian Rhythms of DNA Synthesis and Expression of c-myc Gene in Eca-109 Cells of Human Oesophageal Cancer

为了探讨人食道癌E ca-109细胞DNA合成与c-m yc基因表达的近日节律以及异鼠李素对二者的影响作用,达到有效治疗肿瘤的目的。我们用3H-T dR掺入实验和流式细胞仪检测在24 h内不同时间点异鼠李素对E ca-109细胞DNA合成和c-m yc表达的近日节律的影响,其结果用方差分析和Cos inor法进行统计学分析。实验数据显示人食道癌E ca-109细胞株DNA合成和c-m yc基因的表达随近日节律变化,且异鼠李素对DNA合成和c-m yc基因的表达具有抑制作用,并影响其近日节律特征。人食道癌E ca-109细胞的生长与c-m yc基因表达在20:00至0:00达到高峰,且异鼠李素在此时间段作用较强,这为制定肿瘤时辰化疗方案提供了有价值的参考。

This study was focused on the circadian rhythms of DNA synthesis and the expression of c-myc gene in untreated and treated Eca-109 cells in human oesophageal cancer with isorhmnetin. The circadian rhythms of ^3H-TdR incorporation and expression of c-myc gene in untreated and treated Eca-109 cells were measured by 3H-thymidine uptake assay and flow cytometry. The data collected were analyzed by ANOVA and Cosinor method. DNA synthesis and expression of c-myc gene in untreated group varied according to circadian time with statistical significance, the distribution curves of both DNA synthesis and the expression level of c-myc were fit for cosinor changes. The circadian rhythms of DNA synthesis and circadian parameters of c-myc expression in treated Eca-109 cells changed, The circadian parameters of DNA synthesis and expression level of c-myc varied after treatment by isorhmnetin. The effects of isorhmnetin on cell proliferation and c-myc expression reached the highest level from 20：00 to 0： 00. The results provide a guidance for instituting the chemotherapy and chronotherapy of human tumors, when isorhmnetin is for use as anti-cancer agent.