实验性大鼠脑出血灶周围区内细胞因子IL-1β的表达

Expression of IL-1 β in peri-intracerebral hemorrhage foci in rats

目的研究脑出血区白细胞介素1β(IL1β)表达的动态变化,探讨脑出血的病理生理过程,为临床治疗脑出血提供理论依据。方法纹状体内注入胶原酶造模,采用免疫组织化学亲合素生物素过氧化物酶复合物法(ABC)观察细胞因子IL1β在脑出血后4、14、24、72h和7d的表达变化。结果大鼠脑出血后4h病变局部即有明显的IL1β表达,阳性细胞呈胶质细胞形态;至脑出血后14h,IL1β阳性的细胞数量比4h组明显增多(P<0.05),染色更深,细胞形态相似;至24h,IL1β蛋白的表达达到顶峰(P<0.05),阳性细胞密集,染色最深,形态仍然同前。24h以后,IL1β蛋白的表达则逐渐下降,至脑出血72h后局部几乎无IL1β阳性的细胞(P<0.05);一直至脑出血7d时,局部仍未见到IL1β阳性的细胞。所有动物非出血侧纹状体均未见到明显的IL1β阳性细胞。结论IL1β在脑出血区表达增高,脑出血后病变局部可能存在着与病程相关的炎性反应,在一定时间内采取有效的对抗炎性细胞因子的治疗可能对脑出血患者的预后有一定的意义。

Objective To study dynamic changes of expression of interleukin-1 β （IL-1 β ） in the intracerebral hemorrhage （ICH） fuci and to investigate the pathophysiological process after ICH so as to provide theory basis for clinical treatment. Methods Collagenase was injected into the striaturn of the rats to cause bleeding. Immunohistochemical avidin-biotin-peroxidase complex （ABC） method was used to observe the expression of IL-1 β, 4, 14, 24, 72 h and 7 d ＂after rat ICH. Results There was clear IL-1 β immunopositive cells around pathological changes in striatum in 4 h. And the positive cells were glia-like configuration. At 14 h group after rat ICH, the number and the dyeing of IL-1 β immunopositive cells were more dense than that of 4 h group （P 〈0.05）, but the configuration did not significantly change. At 24 h, the number of IL-1 β positive cells was maximal （ P 〈 0. 05 ）. The positive cells, still without the morphologic changes, centralized in foci and appeared the densest dyeing. And ＇after 24 h, the expression of IL-1 β gradually decreased. At 72 h group, there was almost no IL-1 β immunopositive call in and around pathological changes at hemorrhage side （P 〈0.05 ）. There was still no IL-1 β immunopositive cell in foci till 7 d. At non-hemorrhage side striatums of all animals there was no IL-1β immunopositive cells at ＇all time points. Conclusion The expression of IL-1β increases significantly in and around pathological changes ＇after ICH. And the inflammation reaction related to the course of disease probably exists in the location of ICH. Thus, efficient treatment of anti-IL-1β molecules in special time may be significant to the prognosis of ICH.