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ET-1及其受体ETAR在食管癌中的表达及意义 被引量:1

Expression and clinical significance of endothelin-1 and endothelin type A receptor gene in esophageal cancer
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摘要 目的研究内皮素-1(endothelin-1,ET-1)及其受体ETAR和食管癌生长转移的关系。方法采用RT-PCR及Westernblot检测观察食管癌及配对癌旁食管黏膜组织中的ET-1和ETAR的表达,并结合临床病理特征分析其意义。结果ET-1mRNA在食管癌组织中的阳性表达率为82.7%(62/75),在癌旁食管黏膜组织的阳性表达率为60%(45/75),且其在癌组织中的过度表达与肿瘤分化程度、淋巴结转移、临床分期有关系(P<0.05);ETARmRNA在食管癌组织中的阳性表达率为61.3%(46/75),在癌旁食管黏膜组织的阳性表达率为62.7%(47/75),两者比较无显著性差异(P>0.05)。另外,Westernblot检测显示在74.7%(56/75)的食管癌组织中有ET-1蛋白高表达。结论ET-1与食管癌生长转移有密切关系。 Objective To explore the relationship between the ET-1 and the oncogenesis, development of human esophageal cancer. Methods RT-PCR and Western blotting were employed to detect the expression of ET-1 and ETAR in the tissues of esophageal cancer and normal esophageal mucous membrane from 76 esophageal cancer patients admitted by our hospital from March to December in 2002. Results There was positive ET-1 mRNA expression in 82.7% (62/75) of the specimens of esophageal cancer and in 60% (45/75) of normal tissue and the ET-1 mRNA expression had a relation with histological grade, lymph node metastasis and clinical staging of esophageal cancer (P 〈0.05 ). The positive ETAR mRNA expression was found in 61.3% (46/75) of the specimens of esophageal cancer and in 62.7% (47/75) of normal tissue ( P 〈0.05 ). Western blotting showed that 56 of 75 specimens of esophageal cancer had an overexpression of ET-1 protein. Conclusion ET-1 may play an important role in the oncogenesis, development of esophageal cancer.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2005年第23期2314-2316,共3页 Journal of Third Military Medical University
基金 国家自然科学基金资助项目(30170519)~~
关键词 食管肿瘤 内皮素1 逆转录多聚酶链式反应 基因表达 esophageal neoplasm endothelin-1 reverse transcription-polymerase chain reaction
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