p73和PCNA的表达与喉鳞状细胞癌的预后

Expression and prognosis significance of p73 and PCNA in laryngeal squamous cell carcinoma

目的:探讨喉鳞状细胞癌(LSCC)中p73、增殖细胞核抗原(PCNA)基因的表达与患者临床、病理参数和预后的关系.方法:采用免疫组织化学SABC技术检测65例LSCC组织及23例癌旁组织中p73、PCNA基因的表达情况.结果:p73、PCNA蛋白在65例LSCC组织中的表达水平较23例癌旁组织明显增强(均P＜0.05).65例LSCC组织中,p73蛋白在TNM分期Ⅲ～Ⅳ期、远处转移及复发患者中的表达水平较Ⅰ～Ⅱ期、无转移及初发患者表达增强(P＜0.05).PCNA蛋白在TNM分期Ⅲ～Ⅳ期患者、低分化癌、复发患者和有颈淋巴结转移及远处转移患者中的表达水平较Ⅰ～Ⅱ期、高中分化、初发患者和无转移患者中的表达增强(P＜0.05).PCNA蛋白的表达与患者预后明显相关.经Cox比例风险模型进行分析,PCNA表达是患者预后不良的重要指标(其相对危险度RR=2.43,P＜0.05).LSCC组织中p73、PCNA的基因表达存在正相关性(P＜0.05).结论:p73蛋白在LSCC的发生、发展过程中可能扮演了癌基因的角色.PCNA蛋白的表达与LSCC的危险性存在密切关系,可作为判断LSCC患者预后的重要指标.LSCC中p73、PCNA的表达存在正相关关系.

Objective：This study was designed to investigate the expressionof p73 and Prolife Rating Cell Nuclear Antigen（PCNA） and their association with clinical,histologic characteristics and prognosis in LSCC. Method： p73 protein and PCNA protein were examined by using immunochistochemical SABC staining method in 65 cases of LSCC and in 23 adjacency carcinoma cases. Result：p73 protein and PCNA protein in LSCC shows stronger expression than that in adjacency carcinoma,which is statistically significant（ P 〈0.05）, p73 protein positive expression shows stronger in stage Ⅲ～Ⅳ of LSCC than that in stage Ⅰ～Ⅱ （ P 〈0.05） Fit more often appears in recurrent cases than in primary cases（ P〈0.05）. And p73 protein positive expression with distant metastasis was stronger than that in LSCC without distant metastasis（ P 〈0.05）. PCNA protein positive expression was stronger in stage Ⅲ～Ⅳ of LSCC than that in stage Ⅰ～Ⅱ （ P 〈0.05） ; it more often appears in recurrent cases than in primary cases（ P d0.05）. PCNA protein positive expression more frequently appears in poor differentiation of LSCC, compared to well/mediate differentiation（ P 〈0.05）,PCNA positive expression in cases with cervical lymph node metastasis and distant metastasis was stronger than that without metastasis（ P 〈0.05）. PCNA expression had a significantly stronger in patients whose survival time over 5 years than that in patients whose survival time less than 5 years（ P 〈0.05）. Analysing with multivariate Cox, the expression of PCNA is an important marker of poor prognosis（RR= 2.43, P 〈0.05）. Conclusion.. p73 protein positive expression may play a part in LSCC progression and function as an oncogene in the development of LSCC. PCNA positive expression was closely linked with the risk of LSCC; it can be an important predictor on evaluating the prognosis of LSCC. There was positive correlation existing between p73 protein and PCNA protein expression in LSCC.