金丝桃素联合放射线照射对喉癌细胞Hep-2的影响

Effects of hypericin associated with radiotherapy on human laryngeal squamous cell carcinoma strain Hep-2

目的:探讨金丝桃素(HY)联合放射线照射对喉癌细胞Hep-2的影响。方法:体外培养的喉癌细胞Hep-2分别给予0、1、2、3μg/ml终浓度的HY,加药1h后,给予5Gy的放射线照射(HY加放射组);另取喉癌细胞Hep-2分别给予5、10、20、25μg/ml终浓度的HY,不给予放射线照射(单纯HY组);各实验组均设空白对照。继续培养48h后,应用显微镜、MTT法和流式细胞仪(FCM)分析HY加放射组和单纯HY组对喉癌细胞Hep-2的影响。结果:光镜下可见喉癌细胞生长密集,细胞呈梭型或多角型,细胞彼此之间相接触,放射后的喉癌细胞主要改变为部分细胞肿胀,HY和喉癌细胞共同经放射线作用后,在HY低剂量时即可见细胞数量明显减少,肿胀细胞比例明显增多,高剂量时可见细胞坏死。MTT结果显示放射后HY可显著抑制喉癌细胞的生长;FCM分析表明放射后HY可将喉癌细胞阻滞在G0/G1期,并诱导喉癌细胞凋亡。HY单独应用,对喉癌细胞的抑制作用相对较弱。结论:HY联合放射线照射可有效地抑制喉癌细胞的增殖,并可诱导喉癌细胞凋亡。

Objective：To study effects of hypericin associated with radiotherapy on human laryngeal squamous cell carcinoma strain Hep-2. Method： Using techniques of tumor cells culture in vitro, Hep 2 cells were exposed to different concentration hypericin as 0, 1.0, 2.0, 3.0 μg/ml, then 5 Gy radiation were given after an hour. Other groups Hep-2 culture cells were also exposed to different concentration hypericin as 5.10.20.25 μg/ml, and no radiation were given. In all groups, contrast group was set up. And 48 hours later, growth characteristics of Hep-2 were studied by morphological observation, MTT assay and flow cytometry. Result： In normal contrast group, Hep-2 cells grew intensively and contacted with each other. And cellular swelling was found when Hep-2 cells were only treated with radiotherapy. When ttep-2 cells were treated with both Hypericin and radiotherapy, more cells swelled and cell number declined greatly. In higher dose Hypericin group, necrosis could be found. MTT assay showed the Hep2 ceils growth was significantly inhibited when treated with both hypericin and radiotherapy. Flow cytometry showed hypericin could block cell growth at G0/G1 phase and induced laryngeal cells into apoptosis whether with radiotherapy or not. But Hep-2 cells only treated with hypericin could not get same powerful effect as combination with radiotherapy. Conclusion：H uman laryngeal squamous cell carcinoma strain Hep-2 can be inhib ited and induced into apoptosis by treated with hypericin combination with radiotherapy.