摘要
A new method for the determination of DNA was developed with azocarmine G(AG) in the presence of cetyltrimethylammonium bromide(CTAB) by the resonance light scattering (RLS) technique. The synthetic samples were determined with satisfactory results, and the reaction mechanism was also studied. The results show that underthe optimum conditions, the weak RLS signal of AG can be enhanced by DNA, which results from the formation of a new ternary complex AG-CTAB-DNA with large size. Moreover, the enhanced RLS intensity at 552 nm is directlyproportional to the concentration of DNA in the range of 01.0 μg/mL for fish sperm DNA (fsDNA) and 01.5 μg/mLfor calf thymus DNA (ctDNA). Based on this, a new assay of DNA can be established. The detection limits (3σ) are 2.1 ng/mL for fsDNA and 2.2 ng/mL for ctDNA, respectively.
A new method for the determination of DNA was developed with azocarmine G（AG） in the presence of cetyltrimethylammonium bromide（CTAB） by the resonance light scattering （RLS） technique. The synthetic samples were determined with satisfactory results, and the reaction mechanism was also studied. The results show that under the optimum conditions, the weak RLS signal of AG can be enhanced by DNA, which results from the formation of a new ternary complex AG-CTAB-DNA with large size. Moreover, the enhanced RLS intensity at 552 nm is directly proportional to the concentration of DNA in the range of 0 - 1.0 μg/mL for fish sperm DNA （fsDNA） and 0 - 1.5μg/mL for calf thymus DNA （ctDNA）. Based on this, a new assay of DNA can be established. The detection limits （3σ） are 2. 1 ng/mL for fsDNA and 2.2 ng/mL for ctDNA, respectively.
基金
Project(20475065)supportedbytheNationalNaturalScienceFoundationofChina
