铁离子沉积与心脏移植急性排斥反应相关性研究

Iron accumulation in acute cardiac allograft rejection in mice

目的:探讨铁离子在心脏移植急性排斥反应的作用。方法:建立小鼠颈部异位心脏移植模型,实验分为同系移植组(C57BL/6→C57BL/6)和同种异体移植组(BALB/C→C57BL/6),普鲁士蓝染色观察铁离子在心肌组织的沉积情况,免疫组化法观察HO-1在心肌组织的表达。结果:铁离子在浸润的巨噬细胞中沉积,HO-1主要在浸润的炎性细胞中表达,两者随急性排斥反应的加重表达上调。结论:铁离子沉积与心脏移植急性排斥反应的病理过程相关,且可作为心脏移植急性排斥反应的监测指标。

AIM： To investigate the significance of iron in acute cardiac allograft rejection in mice. METHODS： The model of cervical heterotopic heart transplantation in mice was established. Two groups were studied, including control group （C57BL/6→C57BL/6） and experimental group （BALB/c→C57BL/6）. Beating of the grafted heart was Palpated everyday. At day 1, 3, 5, 7 ＇after transplantation, the grafted hearts were obtained. The grade of acute rejection was estimated by observing the histological sections with HE staining. Prussian blue staining was employed to detect the deposits of iron in myocardium. The expression of HO - 1 proteins in myocardium was measured by immunohistochemistry technique. RESULTS： Prussian blue staining of allograft tissue revealed an accumulation of iron primarily in infiltrating macrophages. Iron was increased in cardiac allografts with acute rejection in mice. Graft cellular infiltrates were identified as the principal source of HO - 1 expression in allograft by immunohistochemistry. CONCLUSIONS： These data suggest that iron may play an important role in acute cardiac allograft rejection. Determination of iron deposits may help estimate the grade of acute cardiac allograft rejection.