腺病毒介导的双自杀基因对胆管癌细胞的体外杀伤作用

Antitumor effects of HSV - tk and cytosine deaminase double suicide gene mediated by adenovirus on cholangiocarcinoma cells in vitro

目的:研究CD、HSV-TK融合基因对胆管癌细胞QBC939生长的抑制作用。方法:构建了含CD、HSV -TK融合基因的重组腺病毒载体,体外转染胆管癌细胞株QBC939,并与单基因转染对照,观察其对胆管癌细胞的杀瘤活性。结果:CD、HSV-TK融合基因经体外转染可在人胆管癌细胞QBC939中高效表达。与单一自杀基因转染组对照,双基因组对前体药物的敏感性增强,表现出更强的抗肿瘤作用,具有更明显的旁观者效应(BSE)。结论:CD/ HSV-TK双自杀基因对胆管癌细胞具有更强的抗肿瘤作用,是一种较单自杀基因治疗更合理的研究方向。

AIM： To study the antitumor effects of HSV - tk and cytosine deaminase double suicide gene system on cholangiocarcinoma cells in vitro. METHODS： Recombinant adenoviras vector carting HSV - tk and cytosine deaminase double suicide gene was coustructed and was trangected into QBC939 huamn cholangiocarcinoma cells. The antitumor effects were observed in vitro in comparison with a single suicide gene system. RESULTS： By transfection, HSV - tk and （or） cytosine deaminase double suicide gene gained high expression in tumor cells. Compared with single suicide gene system, this double gene system printed higher sensitivity, stronger antitumor effect and bystander effect. CONCLUSION： HSV - tk and cytosine deaminase double suicide gene system has a powerful antitumor effect on cholangiocarcinema cells.