宫颈癌抗独特型单克隆抗体的制备和鉴定

Production and identification of monoclonal anti-idiotypic antibody against uterine cervical cancer

目的:制备宫颈癌抗独特型单克隆抗体并鉴定其抗原模拟特性。方法和结果:以识别小鼠和人宫颈癌相关抗原分子共同表位的单克隆抗体AU14-1(AB1)为免疫原在含免疫反应剂的无血清培养液中致敏小鼠脾细胞, 并将其与SP2/0融合,经筛选和克隆化,建成一株能分泌抗独特型单克隆抗体(AB2)的杂交瘤细胞系。AB2的抗原模拟特性经ELISA、结合和竞争抑制试验以及免疫组化染色,表明为AB2Β具有宫颈癌细胞膜表面抗原的“内影像”。结论:获得一株具有宫颈癌抗原内影像的抗独特型单克隆抗体。

AIM： To preduce monoclonal anti- idiotypic antibody against uterine cervical cancer and identify its antigenie mimicry properties. METHODS anti RESULTS： Mouse splenocytes were immunized in serum- free medium containing immunoaetive reagents by using monoclonal antilxody AU14- 1 （ Ab1 ） reognizing common epitopes shared with both mouse and human uterine cervical cancer - associated antigen molecule as immunogen. The in vitro immunized lymphoeytes were fused with murine myelnma SP2/O. Hyhridoma cell line secreting monoclonal anti - idiotypie antibody （Ab2 ） was established by screening and cloning. The identification of antigenic mimicry properties of Ab2 with ELISA, eombinding and competing inhibition assays, and immunohistochemistry staining showed that this mouoclonal anti - idiotypic antibody was Ab2,3, which carried an “internal image” of cervical cancer cell memhrane surface antigen. CONCLUSION： A monoclonal anti- idiotypic antibody with an “internal image” of cervical cancer antigen was obtained.