摘要
目的:探讨心肌匀浆上清液对大鼠骨髓间质干细胞(MSCS)诱导分化的影响。方法:体外分离培养大鼠MSCS,检测纯度。于原代培养第3 D加入自体心肌匀浆上清液持续作用1周。3周后,观察细胞形态,采用免疫细胞化学检测肌球蛋白重链和心肌特异性肌钙蛋白-T,RT-PCR方法检测NKX2．5、Α-MHC和ANP基因的表达。结果:大鼠MSCS经诱导后,表达肌球蛋白重链、心肌特异性肌钙蛋白-T和NKX2．5、Α-MHC基因,但未表达ANP基因, 也未观察到肌管和闰盘样结构。结论:心肌匀浆上清液对MSCS具有定向诱导分化作用,但诱导不完全,具体机制仍需深入研究。
AIM： To investigate the effects of myocardial extracts of rats on transdifferentiation of bone marrow - derived mesenchymal stem cells （MSCs）. METHODS： After establishing stable culture methods for rat MSCs, they were induced by high level of myocardial extracts on day 3 of primary culture for 1 week. Immunocytochemistry technique was used to detect MHC and troponin - T, and the expression of Nkx2.5, α - MHC and ANP genes were identified by RT- PCR technique 3 weeks later. RESULTS： MSCs of rats expressed cardiac MHC and troponin- T proteins, Nkx2.5 and α- MHC genes after induction. However, they neither expressed ANP gene nor formed myotubes and intercalated disk. CONCLUSION： Myocardial extracts can induce MSCs to transdifferentiate into cardiomyocytes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第12期2466-2469,共4页
Chinese Journal of Pathophysiology
关键词
骨髓
干细胞
细胞分化
大鼠
心肌提取液
Bone marrow
Stem cells
Cell differentiation
Rats
Myocardial extracts
