摘要
目的:探讨心肌匀浆上清液对大鼠骨髓间质干细胞(MSCS)诱导分化的影响。方法:体外分离培养大鼠MSCS,检测纯度。于原代培养第3 D加入自体心肌匀浆上清液持续作用1周。3周后,观察细胞形态,采用免疫细胞化学检测肌球蛋白重链和心肌特异性肌钙蛋白-T,RT-PCR方法检测NKX2.5、Α-MHC和ANP基因的表达。结果:大鼠MSCS经诱导后,表达肌球蛋白重链、心肌特异性肌钙蛋白-T和NKX2.5、Α-MHC基因,但未表达ANP基因, 也未观察到肌管和闰盘样结构。结论:心肌匀浆上清液对MSCS具有定向诱导分化作用,但诱导不完全,具体机制仍需深入研究。
AIM: To investigate the effects of myocardial extracts of rats on transdifferentiation of bone marrow - derived mesenchymal stem cells (MSCs). METHODS: After establishing stable culture methods for rat MSCs, they were induced by high level of myocardial extracts on day 3 of primary culture for 1 week. Immunocytochemistry technique was used to detect MHC and troponin - T, and the expression of Nkx2.5, α - MHC and ANP genes were identified by RT- PCR technique 3 weeks later. RESULTS: MSCs of rats expressed cardiac MHC and troponin- T proteins, Nkx2.5 and α- MHC genes after induction. However, they neither expressed ANP gene nor formed myotubes and intercalated disk. CONCLUSION: Myocardial extracts can induce MSCs to transdifferentiate into cardiomyocytes.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第12期2466-2469,共4页
Chinese Journal of Pathophysiology
关键词
骨髓
干细胞
细胞分化
大鼠
心肌提取液
Bone marrow
Stem cells
Cell differentiation
Rats
Myocardial extracts