慢性乙型肝炎患者α干扰素治疗应答与肿瘤坏死因子及白介素10单核苷酸多态性的相关性

Influence of single nucleotide polymorphisms of TNF and interleukin-10 genes on response to interferon-α therapy in patients with chronic hepatitis B infection

目的:探讨慢性乙型肝炎患者α干扰素治疗应答与肿瘤坏死因子(TNF)及白介素10(IL-10)单核苷酸多态性的相关性。方法:对43例α干扰素抗病毒治疗的慢性乙型肝炎患者治疗结束后随访6个月;以随访完成时完全应答和部分应答者为应答组,无应答者为无应答组;随访完成后应用TaqMan技术对43例患者TNF-α-238、TNF-α-308、TNF-β(LTA)+723、IL-10-592位点进行基因型分析。结果:43例患者中α干扰素治疗应答组26人,无应答组17人。应答组和无应答组之间TNF-α-238、TNF-α-308、TNF-β+723基因型的分布无显著差异。干扰素治疗应答组IL-10-592位点等位基因A的出现频率高于无应答组(P<0.05)。结论:慢性乙型肝炎患者干扰素治疗应答与TNF-α-238、TNF-α-308、LTA+723位点单核苷酸多态性可能无关,而与IL-10-592位点等位基因A可能有关。

Objective： To study the influence of single nucleotide polymorphisms （SNPs） of tumor necrosis factor（TNF） and interleukin-10（IL 10） genes on response to interferon-α therapy in patients with chronic hepatitis B（HBV） infection. Methods： A total of 43 patients with chronic HBV infection undergoing interferon α therapy were followed up for 6 months,and were divided into responding group（n= 26） and non responding group（n= 17）. Genomic DNA was extracted from each individual and genotyped using TaqMan assay for 2 promoter SNPs in TNF-α gene （TNF-α-238 and -308）, 1 in TNF-β（LTA） gene （LTA ＋723）, and 1 in IL- 10 gene （IL-10-592）. Results： There were no significant differences between responders and non-responders in the distribution of the genotypes or alleles of the SNPs TNF-α-238,TNF-α-308 and LTA＋723. However, the A allele of IL-10-592 was more frequent in the responders than in the non responders（P〈0.05）. Conclusion： There may be no links between the TNF genetic polymorphisms and response to interferon α therapy in HBV patients,but the A allele of IL-10-592 may play a role in the response of interferon α treatment in chronic HBV infection.