快速叶绿素荧光诱导动力学分析在光合作用研究中的应用

Application of the Fast Chlorophyll Fluorescence Induction Dynamics Analysis in Photosynthesis Study

JIP-测定(JIP-test)是以生物膜能量流动为基础建立的分析方法。利用该方法可以获得有关光系统II的大量信息。文章介绍了快速叶绿素荧光诱导动力学曲线的定义、数据分析方法及相关参数的意义,并举例说明如何利用该方法分析不同环境条件对光合机构主要是PSII的供体侧、受体侧及PSII反应中心的影响。

The changes in PSⅡ performance of the photosynthetic apparatus caused by environmental stress or senescence have been explored widely by applying the chlorophyll fluorescence technique, mainly by using the modulated fluorometer. In recent years, an alternative approach via analyzing chlorophyll a fluorescence transient using the continuous excitation fluorometer has been developed, which offers more information that cannot be obtained by the modulated fluoremetry. The JIP-test based on the theory of energy fluxes in biomembranes has been widely used to analyze the chlorophyll a fluorescence kinetics transient helping researchers to have a deeper insight into the primary photochemical reaction in the photosyntheticapparatus. In this paper, the chlorophyll a fluorescence transient and its significance, the parameters involved and their significance in the JIP-test, and the application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study are introduced combining some results of the author＇s.