摘要
目的:建立茅莓总皂苷的含量测定方法。方法:通过对样品采用不同溶剂及不同提取方法所得的提取液显色后,以分光光度法测定总皂苷的含量。结果:本法测定的线性范围为22-176μg(r=0．9997);平均回收率为102．1％,RSD=2．82％ (n=6);样品以70％乙醇回流提取所得的总皂苷含量最高。结论:该方法灵敏、可靠,显色后的样品比较稳定,可用于茅莓药材的质量控制及品质评价。
Objective： To establish a method for content determination of total saponins in Rubus parvifolius L.. Methods： Total saponins was determined by UV - VIS spectrophotometry after different extracts of the sample had been coloured. Results ： The method was linear in the range of 22- 176μg （r =0. 9997 ） ,and the average recovery was 102.1%, RSDwas 2.82% （n = 6 ）. The content of total saponins was highest by the method of 70% ethanol reflux. Conclusion ： The sample is stable after coloured. The methods sensitive and reliable, which may be used for the quality control of Rubus parvifolius L..
出处
《重庆医科大学学报》
CAS
CSCD
2005年第6期854-856,共3页
Journal of Chongqing Medical University
基金
重庆市科技攻关重大专项(2004-06)。
关键词
茅莓
总皂苷
含量测定
分光光度法
Rubus parvifolius L.
Total saponins
Content determination
UV -VIS spectrophotometry
