胃癌中DNA聚合酶β的突变及与幽门螺杆菌感染的关系

DNA polymerase β gene mutation in gastric carcinoma and the relationship with H.pylori infection

目的:研究胃癌组织中DNA聚合酶β基因(polβ)的突变情况及与幽门螺杆菌感染的关系。方法:提取总RNA反转录合成cDNA,经PCR扩增后以SSCP检测其变异情况。用PCR方法检测胃癌及癌旁组织标本中幽门螺杆菌的感染情况。结果:32例胃癌组织标本中有7例polβ-SSCP异常,突变率为21.9%;而对应的32例癌旁组织PCR-SSCP结果中未见异常。H.Pylori-DNA阳性的15例,H.Pylori-DNA阳性率为46.9%(15/32);对应癌旁组织的检测结果与胃癌组织完全一致。polβSSCP阳性的标本H.Pylori-DNA也阳性。结论:胃癌组织存在polβ基因突变;这种基因突变可能与胃癌的发生、发展相关。幽门螺杆菌的感染与胃癌组织polβ突变可能存在一定的相关关系。

Purpose： To detect DNA polymerase β gene （polβ） mutations in human gastric cancer specimens and the relationship between H. pylori infectinn and polβ mutation. Methods： Extracting total RNA front gastric carcinoma, corresponding cancer specimens tissues and normal tissues, synthesizing eDNA and then using them as templates proceed PCR, The products of PCR were checked by SSCP. Extracting DNA front the specimen, we could detect the H. Pylori front the tissue of gastric carcinoma and the tissue adjacent to them by PCR. Results： There were 7 abnormal SSCP of 32 gastric carcinoma samples, and the mutation rate was 21.9%, but nothing abnormal was found in the tissues adjacent to the tumor. The results of H. Pylori DNA were positive in 15 samples from 32 gastric carcinoma tissues. Positive rate was 46.9（ 15/ 32）. Detection result of tissues adjacent to tumor was consistent with gastric carcinoma. Comparing polβ SSCP to H. pylori- DNA in gastric carcinoma, we found the positive samples of polβ SSCP correlated with H, pylori-DNA. Conclusions： It is suggested that the polβgene mutation may be associated with carcinogenesis and development of human gastric cancer. H. pylori infection is possildy related to polβ mutation in gastric carcinoma.