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牛肌肉生成抑制素基因真核表达载体的构建及其在COS-7细胞中的表达 被引量：2

Construction of eukaryotic expression vector with cattle myostatin gene and its expression in COS-7 cells
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摘要 将构建的牛pMD 18-T-MSTN克隆载体与真核表达载体pef-dhfr1a酶切,回收牛MSTN目的片段及pef-dhfr1a载体,构建了牛MSTN基因的真核表达质粒pef-dhfr1a-MSTN,然后转染COS-7细胞,将牛MSTN成熟蛋白编码序列在COS-7细胞中进行了表达。提取转染细胞的总RNA,采用RT-PCR和Western-blotting方法,分别从mRNA水平和蛋白质水平上检测到了牛MSTN基因在COS-7细胞中的表达产物,证明已经成功构建出该基因的真核表达载体。 In order to express encoding sequence of the cattle myostatin gene in eukaryotic system, a cloning plasmid and a pef-dhfrla vector was digested and then retrieved, respectively. The constructed eukaryotic expression plasmid pef-dhfrla-MSTN was induced by liposome. The cattle myostatin gene encoding a protein of 375 amino acids was expressed in COS-7 cells. The RT-PCR in mRNA level and Westernblotting analysis in protein level confirmed that the eukaryotic expression plasmid pef-dhfrla-MSTN was constructed successfully.
作者 吕文发 赵静 姚淑珍 王军
机构地区 吉林农业大学动物科技学院
出处 《中国兽医科技》 CSCD 北大核心 2005年第12期997-999,共3页 Chinese Journal of Veterinary Science and Technology
基金 国家自然科学基金项目(30500366)
关键词 牛 肌肉生成抑制素基因 真核表达 COS-7细胞 cattle myostatin gene eukaryotic expression COS-7 cell
分类号 S859.79 [农业科学—临床兽医学] Q786 [生物学—分子生物学]
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参考文献10

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二级参考文献8

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共引文献4

同被引文献27

  • 1王全喜,红海,芒来.不同品种马肌肉抑制素基因的克隆与PCR-RFLP多态性分析[J].中国草食动物,2005,25(4):11-13. 被引量：4
  • 2吕文发,赵静,袁天祥.西门塔尔牛肌肉生成抑制素基因编码序列的克隆与序列分析[J].中国牛业科学,2006,33(1):19-20. 被引量：2
  • 3Lee S J, Mcpherron A C. Regulation of myostaton activity and muscle growth [J]. Proc. Natl. Acad. Sci. USA,2001(98):9306-9311
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  • 10Hughes S M. Running without regulators. Nature,1992(360):536-537

引证文献2

二级引证文献3

中国兽医科技
2005年 第12期

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