仓鼠胰腺癌原位模型的建立及生物学特性

Establishment of orthotopic model of hamster pancreatic cancer and its biologic characteristics

目的建立仓鼠胰腺癌原位模型,并研究其生物学特性.方法将仓鼠胰腺癌细胞株pGHAM-1接种于仓鼠胰腺背膜下,分别于第7,14,21,28日各剖杀10只,肉眼及光学显微镜下观察原位肿瘤的生长、淋巴结转移及肝转移,同时观测腹水量.结果仓鼠胰腺癌原位模型成活率达95%,生长迅速.接种后第7,14,21,28日原位肿瘤大小分别为(16±6),(173±44),(369±87),(974±226)mm3.第21日,毛细淋巴管内可见癌细胞团,部分淋巴结有癌细胞转移,可见少量淡血性腹水.第28日,淋巴结阳性率进一步增加,可见血性腹水,量明显增多;部分仓鼠发生了肝转移.结论仓鼠胰腺癌细胞株pGHAM-1原位模型建立方法简便,易复制,而且肿瘤保持了胰腺癌的生物学特性,是理想的胰腺癌研究模型.

AIM： To establish an orthotopic model of hamster pancreatic cancer and to analyze its biologic characteristics. METHODS： Pancreatic cancer cell line pDHAM-1 of hamster was inoculated under pancreatic membrane. At 7th, 14th, 21st, and 28th day, a group of 10 animals were sacrificed to observe the growth of orthotopic cancer, lymph nodes metastasis, liver metastasis and the quantity and quality of ascites. RESULTS： Orthotopic model of hamster pancreatic cancer had a high survival rate （95%） and grew rapidly. The orthotopic tumors were measured （16 ± 6）, （173 ±44）, （369 ±87）, （974 ±226） mm^3 respectively at the 7th, 14th, 21st and 28th day. At the 21st day, cancer cells were observed in lymphatic capillaries and metastasis in some lymph nodes and hydremic ascites was detected. At the 28th day, the positivity rate of lymph nodes and the amount of bloody ascites increased. Liver metastasis was detected in some of the hamsters. CONCLUSION： The establishment of orthotopic model of hamster pancreatic cancer cells pGHAM-I is simple and easily copied, and maintains the biologic characteristics of pancreatic carcinoma, which is an ideal research model of pancreatic carcinoma.