超声引导下经皮兔肝内VX2肿瘤接种方法初探及其超声评价

Establishment of rabbit liver cancer model by implanting VX2 tumor under ultrasound guidance and its sonographical evaluation

目的改进兔肝VX2肿瘤模型的制作使之更适合肝癌微创治疗的实验研究,同时评价超声检查在监测接种后肿瘤成瘤过程中的价值.方法新西兰大白兔15只,在超声引导下将瘤块(约0.5 mm×0.5 mm×0.5 mm)2～4个接种在兔肝左、右叶,于接种后20、36 d行超声检查,同期剖腹观察接种情况.结果15只兔接种30个肿瘤,16个(53%)接种成功,接种时间为15～40 min,接种位置与是否成瘤无关,接种并发症少;超声与病理检查结果有较好的一致性.结论改进后模型制作更简单,费时少,创伤小,并发症少.接种后要动态连续行超声检查观察成瘤过程.

Objective To improve the establishment of rabbit model with VX2 liver tumor and assess sonographical value in monitoring the tumor growth. Methods Fifteen New Zealand white rabbits were implanted with VX2 tumor in two liver lobes under ultrasound guidance percutaneously. Ultrasound examinations were performed at twenty days and thirty six days after implantation. At the same time exploratory laparotomy was performed. Results Among 30 implanted tumors of 15 rabbits, 16（53%） tumors were successfully implanted with few complications. It took 15 to 40 minutes to implant tumors in one rabbit. Implantation rates of left lobe and right lobe were 53% and 50% respectively, and there was no significant difference. Ultrasound examination could monitor tumor growth well. Conclusions After the improvement, the establishment of model was easier, less time-consuming, minimally invasive and caused few complications than other implantation methods. Serial ultrasound examinations should be performed in monitoring the tumor growth.