白细胞介素-8在妊娠早期小鼠子宫内膜的表达及对胚泡着床的影响

Expression of interleukin 8 in mouse endometrium during early pregnancy and its effect on blastocyst implantation

目的:研究白细胞介素-8(IL-8)在妊娠早期小鼠子宫内膜的表达及其对小鼠胚泡着床的影响。方法:用免疫组化法及图像分析技术对IL-8在妊娠1-6 d小鼠子宫内膜的表达进行定位和测定;子宫角注入IL-8抗体,探讨IL-8对胚泡着床的影响。结果:在子宫内膜腔上皮,IL-8主要定位于细胞的游离面,妊娠1 d阳性反应最弱,4 d表达最强,5 d有所下降,6 d又开始增强; 在子宫内膜腺上皮,妊娠4 d表达最弱,5 d和6 d最强;在子宫内膜的基质细胞,随妊娠天数增加,IL-8的表达逐渐增强。IL-8Ab 明显抑制小鼠胚泡着床。结论:IL-8在妊娠早期小鼠子宫内膜持续表达,并参与胚泡的着床调控过程。

Objective： To study the expression of interleukin-8 （IL-8） in mouse endometrium during early pregnancy and the effect of IL-8 on mouse blastocyst implantation. Metheds： The localization and quantity of IL-8 expression in mouse endometrium on day 1-6 of pregnancy were studied by immunohistochemistry method and image analysis. The effect of IL-8 on the implantation was observed by injecting IL-8 antibodies into uterine horn of mouse. Results： In the luminal epithelium of endometrium, IL-8 was mainly located in the free surface. The expression of IL-8 was the weakest on day 1 and strongest on day 4, then decreased on day 5, increased again on day 6. In the glandular epithelium of endometrium, the expression of IL-8 was the weakest on day 4, and the strongest on day 5 and day 6. In endometrium stroma cells, the expression of IL-8 was reinforced gradually with the pregnant progress. IL-8 antibody uterine horn injection showed significant repressing effect on mouse blastocyst implantation. Conclusion： These results suggest that IL-8 is persistently expressed in mouse endometrium during the early pregnancy and participates in the regulation process of mouse blastocyst implantation.