期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

B104CM对早期培养时大鼠O2A祖细胞迁移的促进作用

Promoting effect of B104CM on migration of O2A progenitor in early development of rat
下载PDF
导出
摘要 目的:研究B104CM对体外培养条件下的少突胶质前体细胞的促迁移作用。方法:取新生SD大鼠大脑皮质原代混合培养少突胶质前体细胞,B104神经胶质瘤细胞株条件培养液增殖并纯化,琼脂糖滴迁移实验观察B104CM对少突胶质前体细胞迁移的影响。结果:B104CM实验组可以促进少突胶质前体细胞的迁出,迁移速度有时段上的差别。结论:B104CM对体外培养的少突胶质前体细胞有增殖作用的同时,还可促进其迁移。 Objective： To study the promoting effect of B104CM on migration of O2A progenitors in vitro. Methods： The O2A progenitor obtained from neonatal SD rat cortex was primarily cultured and was allowed to proliferate in B104 conditioned medium. The promoting effect of B104CM on migration of O2A progenitors was observed by agarose drop assay method. Results： B104CM accelerated migration of O2A progenitors and the migrating speed was different during different time periods. Conclusion： B104CM can promote proliferation and migration of O2A progenitors in vitro.
作者 付元山 汪洋 刘才栋 沈馨亚 王滨
机构地区 大连医科大学解剖学教研室 复旦大学医学院解剖学教研室
出处 《解剖学杂志》 CAS CSCD 北大核心 2005年第6期644-646,共3页 Chinese Journal of Anatomy
关键词 少突胶质前体细胞 细胞迁移 B104CM 02A祖细胞 大鼠 促进作用 oligodendrocyte cell migratiom B104CM O2A progenitor rat
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献14

  • 1Berry M,Bannister LH,Standring SM.Nervous system.In:Williams PL eds.38th Gray's anatomy.Churchill Livingstone:Pearson Professional Limited,1995.921-944.
  • 2Reynolds R,Wilkin GP.Development of macroglial cells in rat cerebellum Ⅱ:an in situ immunohistochemical study of oligodendroglial lineage from precursor to mature myelinating cell.Development,1988,102(2) :409-425.
  • 3Levison SM,Goldman JE.Multipotential and lineage restricted precursors coexist in the mammalian perinatal subventricular zone.J Neurosci Res,1997,48(2):83-94.
  • 4Agresti C,Meomartini ME,Amadio S,et al.ATP regulates oligodendrocyte progenitor migration,proliferation,and differentiation:involvement of metabotropic P2 receptors.Brain Res Brain,2005,48(2) :157-165.
  • 5Kakinuma Y,Saito F,Ohsawa S,et al.A sulfatase regulating the migratory potency of oligodendrocyte progenitor cells through tyrosine phosphorylation of beta-catenin.J Neurosci Res,2004,77(5):653-661.
  • 6付元山,汪洋,刘才栋,沈馨亚,王滨.新生大鼠少突胶质前体细胞的培养[J].解剖学杂志,2005,28(6):718-721. 被引量：1
  • 7Varanl J,Orr W,Ward PA.A comparison of the migration patterns of normal and malignant cells in two assay systems.Am J Pathol,1978,90(1):159-171.
  • 8Asakura K,Hunter SF,Rodriguez M.Effects of transforming growth factor-beta and platelet-derived growth factor on oligodendrocyte precursors:insights gained from a neuronal cell line.J Neurochem,1997,68(6) :2281-2290.
  • 9Raff MC,Lillien LE,Richardson WD,et al.Platelet-derived growth factor from astrocytes drives the clock that times oligodendrocyte development in culture.Nature,1988,333 (6173) :562-565.
  • 10Mckinnon RD,Matsui T,Dubois-Dalcq M,et al.FGF modulates the PDGF-driven pathway of oligodendrocyte development.Neuron,1990,5(5) :603-614.

二级参考文献14

  • 1姜文跃,沈馨亚,苏清芬,彭裕文,郑思竞,张素春,汪洋,王子美,吴铁南.体外培养中少突胶质细胞在立体网架上的迁移[J].神经解剖学杂志,1993,9(1):63-66. 被引量：11
  • 2薛庆善,郭畹华.大鼠大脑皮质星形胶质细胞的体外培养及其促神经突起生长作用研究[J].神经解剖学杂志,1996,12(2):151-155. 被引量：30
  • 3Knapp P E,Bartlett W P,Skoff R P.Cultured oligodendrocytes mimic in vivo phenotypic characterisitics:Cell shape,expression of myelin-specific antigens and membrane prodution.Dev Biol,1987,120:356-365.
  • 4Agresti C,Meomartini ME,Amadio S,et al.ATP regulates oligodendrocyte progenitor migration,proliferation,and differentiation:involvement of metabotropic P2 receptors.Brain Res Brain,2005,48(2):157-165.
  • 5Kakinuma Y,Saito F,Ohsawa S,et al.A sulfatase regulating the migratory potency of oligodendrocyte progenitor cells through tyrosine phosphorylation of beta-catenin.J Neurosci Res,2004,77(5) :653-661.
  • 6Zhang SC,Ge B,Duncan ID.Adult brain retains the potential to generate oligodendroglial progenitors with extensive myelination capacity.Proc Natl Acad Sci USA,1999,96(7) :4089-4094.
  • 7Reynolds R,Wilkin GP.Development of macroglial cells in rat cerebellum Ⅱ:an in situ immunohistochemical study of oligodendroglial lineage from precursor to mature myelinating cell.Development,1988,102(2) :409-425.
  • 8Levison SM,Goldman JE.Multipotential and lineage restricted precursors coexist in the mammalian perinatal subventricular zone.J Neurosci Res,1997,48(2) :83-94.
  • 9Armstrong RC.Isolation and characterization of immature oligodendrocyte lineage cells.Methods,1998,16(3) :282-292.
  • 10Barres BA,Raff MC,Gaese F,et al.A crucial role for neurotrophin-3 in oligodendrocyte development.Nature,1994,367(6461) :371-375.
解剖学杂志
2005年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部