Sorcin过表达——白血病多药耐药的一种新机制

Sorcin overexpression: novel mechanism associated with multidrug-resistance in human leukemia cells

目的研究sorcin基因与白血病多药耐药的相关性。方法用NorthernBlot和WesternBlot方法,检测人白血病耐药细胞株K562/A02及其野生型细胞株K562中sorcin基因和蛋白表达水平的差异。设计针对sorcin基因的反义寡核苷酸,通过脂质体将其导入K562/A02细胞,通过WesternBlot方法检测转染前后细胞内sorcin蛋白表达的变化,运用MTT方法检测转染反义寡核苷酸后细胞对阿霉素敏感性的变化。结果NorthernBlot和WesternBlot结果表明,K562/A02细胞中sorcin表达水平明显高于K562敏感细胞。将sorcin基因反义寡核苷酸转入K562/A02细胞后,细胞内sorcin蛋白表达明显下降,对阿霉素的敏感性提高。结论作为一种耐药相关基因,sorcin参与了白血病多药耐药细胞耐药表型的形成,有望成为今后白血病耐药诊断和治疗的新靶点。

Aim To explore the role of sorcin gene on the muhidrug resistance phenotype of human leukemia cell line K562/A02. Methods The difference of sorcin expression level between K562/A02 and its parental cell line K562 were determined using Northern blot and Western blot. An antisense oligodeoxynucleotide （ASODN） targeted sorcin was transfected to K562/ A02 using Oligofectamine. The expression of sorcin was measured using Western blot. The sensitivity of cells to doxorubicin was measured using M3~F assay. Results The sorcin level was significantly higher in K562/A02 cells than in K562 cells . Transfection of K562/A02 cells with ASODN targeted to sorcin reduced the level of sorcin expression and increased the sensitivity of K562/A02 cell to doxorubicin. Conclusion Sorcin, an important gene involved in MDR in leukemia cells, may be a potential target for MDR modulators investigation in the future.