知母有效成分体内外给药对血小板聚集的抑制作用

Inhibitory effects of effective component in Zhimu on platelet aggregation in vitro and in vivo

目的研究知母有效成分(化合物9714)对血小板聚集性的影响。方法采用比浊法测定血小板聚集功能。结果化合物9714代谢产物10-3、10-4、10-5、10-6mol·L-1体外呈浓度依赖性抑制二磷酸腺苷(ADP)、胶原诱导的家兔血小板聚集(P<0.05,P<0.01);化合物971410、20、40mg·kg-1体内(ig)明显抑制ADP、胶原诱导的大鼠血小板聚集,5～7d给药效果较好(P<0.05,P<0.01)。结论化合物9714代谢产物对血小板聚集具有明显的抑制作用。

Aim To study the effects of effective component in Zhimu （compound 9714） on platelet aggregation. Methods Turbidimetry was used to measure platelet aggregation. Results Metabolite of compound 9714 10^-3,10^-4,10^-5 and 10^-6 mol · L^-1 inhibited rabbit platelet aggregation induced by ADP and collagen in vitro, respectively（ P 〈0.05 ,P 〈0. 01 ）. Compound 9714 10, 20 and 40 mg · kg^-1 ig for 5 d to 7 d markedly inhibited platelet aggregation induced by ADP and collagen in rats（ P 〈0. 05 ,P 〈0. 01 ）. Conclusion Metabolite of compound 9714 could inhibit platelet aggregation evidently.